alpha-Melanocyte-stimulating hormone induces hapten-specific tolerance in mice

被引:0
|
作者
Grabbe, S
Bhardwaj, RS
Mahnke, K
Simon, MM
Schwarz, T
Luger, TA
机构
来源
JOURNAL OF IMMUNOLOGY | 1996年 / 156卷 / 02期
关键词
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
alpha-Melanocyte-stimulating hormone (alpha-MSH) is a proopiomelanocortin-derived peptide with known immunoregulatory effects, acting mainly via modulation of cytokine secretion by lymphocytes and monocytes. When applied epicutaneously, alpha-MSH inhibits both induction as well as elicitation of contact hypersensitivity (CHS) responses in mice, We questioned whether systemically administered alpha-MSH leads to the induction of hapten-specific tolerance. For this purpose, mice were injected i.v. with 75 mu g/kg synthetic bioactive alpha-MSH 2 h before sensitization (day 0) or challenge (day 6) with the hapten, trinitrochlorobenzene (TNCB). Intravenous administration of alpha-MSH 2 h before sensitization or 2 h before challenge resulted in a markedly reduced CHS response. To distinguish between unresponsiveness and tolerance, these mice were sensitized and challenged a second time, but in the absence of alpha-MSH. Mice that had been injected with alpha-MSH before the first sensitization (day 0), but not before challenge (day 6), were also unable to develop a significant CHS response after an additional sensitization and challenge with the same Ag 10 to 14 days later, In contrast, sensitization to the unrelated hapten, dinitrofluorobenzene, was unaffected in these mice, indicating the induction of hapten-specific tolerance by alpha-MSH. Moreover, regional lymph node cells obtained from alpha-MSH-treated mice 5 days after resensitization failed to produce IL-2 in response to trinitrobenzosulfonic acid, the water-soluble analogue of TNCB, whereas lymph node cells from TNCB-sensitized, not alpha-MSH-treated, mice as well as from mice treated with alpha-MSH before challenge readily exhibited trinitrobenzosulfonic acid-specific IL-2 production in this assay, Finally, in vivo tolerance induction by alpha-MSH could be abrogated by the administration of anti-IL 10 Ab at the site of sensitization. These data indicate that alpha-MSH, in addition to its suppressive effect on induction and elicitation of CHS, is able to induce hapten-specific tolerance in mice, Thus, alpha-MSH may be a significant regulatory mediator of cutaneous immune responses in vivo.
引用
收藏
页码:473 / 478
页数:6
相关论文
共 50 条
  • [41] ALPHA-MELANOCYTE-STIMULATING HORMONE INDUCES FIBROBLAST-DERIVED COLLAGENASE/MATRIX METALLOPROTEINASE-1
    KISS, M
    WLASCHEK, M
    MICHEL, G
    LANGE, TS
    SCHARFFETTERKOCHANEK, K
    KEMENY, L
    RUZICKA, T
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 103 (03) : 430 - 430
  • [42] EFFECT OF CORTICOSTEROIDS ON ALPHA-MELANOCYTE-STIMULATING HORMONE AND ADRENOCORTICOTROPIN RELEASE IN RAT
    OLIVER, C
    USATEGUI, R
    VAUDRY, H
    ROZENBERG, I
    MOURRE, AM
    JOURNAL OF ENDOCRINOLOGY, 1976, 68 (02) : 351 - 352
  • [43] Alpha-Melanocyte-Stimulating Hormone (α-MSH) suppresses corneal angiogenesis and inflammation
    Najafi, Sheyda
    Elbasiony, Elsayed
    Zidan, Asmaa A.
    Yin, Jia
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2023, 64 (08)
  • [44] Risks of unregulated use of alpha-melanocyte-stimulating hormone analogues: a review
    Habbema, Louis
    Halk, Anne Berthe
    Neumann, Martino
    Bergman, Wilma
    INTERNATIONAL JOURNAL OF DERMATOLOGY, 2017, 56 (10) : 975 - 980
  • [45] LOCALIZATION OF ALPHA-MELANOCYTE-STIMULATING HORMONE IN RAT-BRAIN AND PITUITARY
    DUBE, D
    LISSITZKY, JC
    LECLERC, R
    PELLETIER, G
    ENDOCRINOLOGY, 1978, 102 (04) : 1283 - 1291
  • [46] The human basophil -: a novel target of the neuropeptide alpha-melanocyte-stimulating hormone
    Boehm, M.
    Raap, U.
    Scholzen, T.
    Mertens, M.
    Brehler, R.
    Kapp, A.
    Luger, T. A.
    EXPERIMENTAL DERMATOLOGY, 2006, 15 (08) : 643 - 643
  • [47] SUBCELLULAR-LOCALIZATION OF ALPHA-MELANOCYTE-STIMULATING HORMONE IN RAT HYPOTHALAMUS
    BARNEA, A
    OLIVER, C
    PORTER, JC
    JOURNAL OF NEUROCHEMISTRY, 1977, 29 (04) : 619 - 624
  • [48] ALPHA-MELANOCYTE-STIMULATING HORMONE STIMULATION OF ALDOSTERONE SECRETION IN HYPOPHYSECTOMIZED RATS
    SHENKER, Y
    VILLAREAL, JZ
    SIDER, RS
    GREKIN, RJ
    ENDOCRINOLOGY, 1985, 116 (01) : 138 - 141
  • [49] DISTRIBUTION AND CHARACTERIZATION OF ALPHA-MELANOCYTE-STIMULATING HORMONE IN RAT-BRAIN
    OLIVER, C
    PORTER, JC
    ENDOCRINOLOGY, 1978, 102 (03) : 697 - 705
  • [50] The isolation and structure of alpha-melanocyte-stimulating hormone from horse pituitaries
    Dixon, Jonathan S.
    Li, Choh Hao
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1960, 82 (17) : 4568 - 4572