A novel protein, MUDENG, induces cell death in cytotoxic T cells

被引：17

作者：

Lee, Mi-Rha ^{[1
]}

Shin, Jin Na ^{[1
]}

Moon, Ae Ran ^{[1
]}

Park, Sun-Young ^{[1
]}

Hong, Gilsun ^{[1
]}

Lee, Mi-Ja ^{[2
]}

Yun, Cheol-won ^{[3
]}

Seol, Dai-Wu ^{[4
,5
,8
]}

Piya, Sujan ^{[1
]}

Bae, Jeehyeon ^{[6
]}

Oh, Jae-Wook ^{[7
]}

Kim, Tae-Hyoung ^{[1
]}

机构：

[1] Chosun Univ, Dept Biochem, Sch Med, Kwangju 501759, South Korea

[2] Chosun Univ, Sch Med, Dept Pathol, Kwangju 501759, South Korea

[3] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[4] Kyungwon Univ, Gachon BioNano Res Inst, Songnam 461700, South Korea

[5] Kyungwon Univ, Dept Life Sci, Songnam 461700, South Korea

[6] Pochon CHA Univ, Sch Med, Grad Sch Life Sci & Biotechnol, Songnam 463836, South Korea

[7] Chosun Univ, Sch Med, Dept Anat, Kwangju 501759, South Korea

[8] Univ Pittsburgh, Sch Med, Dept Surg, Pittsburgh, PA 15261 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 370卷 / 03期

关键词：

MUDENG; cell death; adaptin domain;

D O I：

10.1016/j.bbrc.2008.03.139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A screening system comprised of a randomized hybrid-ribozyme library has previously been used to identify pro-death genes in Fas-mediated apoptosis, and short sequence information of candidate genes from this system was previously reported by Kawasaki and Taira [H. Kawasaki, K. Taira, A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme libraries, Nucleic Acids Res. 30 (2002) 3609-3614]. In this study, we have cloned the full-length of the candidate's open reading frames and found that one of the candidates, referred to as MUDENG (Mu-2 related death-inducing gene), which is composed of 490 amino acids that contain the adaptin domain found in the mu 2 subunit of APs related to clathrin-mediated endocytosis, is able to induce cell death by itself. Ectopic expression of MUDENG induced cell death in Jurkat T cells and HeLa cells. In addition, when MUDENG expression was evaluated by immunohistochemical staining, it was found in most tissues, including the intestine and testis. Furthermore, MUDENG appears to be evolutionary conserved from mammals to amphibians, suggesting that it may have a common role in cell death. Taken together, these results suggest that MUDENG is likely to play an important role in cell death in various tissues. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：504 / 508

页数：5