The promotion of salinomycin delivery to hepatocellular carcinoma cells through EGFR and CD133 aptamers conjugation by PLGA nanoparticles

被引:3
作者
Jiang, Jianxin [1 ]
Chen, Huaiwen [1 ,2 ]
Yu, Chao [1 ]
Zhang, Yingying [3 ]
Chen, Meiyuan [1 ]
Tian, She [1 ]
Sun, Chengyi [1 ]
机构
[1] Affiliated Hosp Guiyang Med Coll, Dept Biliary Hepat Surg, Guiyang, Guizhou, Peoples R China
[2] Second Mil Med Univ, Int Join Canc Inst, Shanghai 200433, Peoples R China
[3] Second Mil Med Univ, Coll Pharm, Shanghai 200433, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
CD133; EGFR; hepatocellular carcinoma; nanoparticles; salinomycin; CANCER STEM-CELLS; TARGETED DRUG-DELIVERY; TUMOR-INITIATING CELLS; GROWTH-FACTOR APTAMER; BREAST-CANCER; PEG NANOPARTICLES; IMMUNOLIPOSOMES; EQUILIBRIUM; MICE;
D O I
10.2217/NNM.15.43
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To develop salinomycin-loaded poly(lactic-co-glycolic acid) nanoparticles conjugated with both CD133 aptamers A15 and EGFR aptamers CL4 (CESN), to target hepatocellular carcinoma (HCC) cells simultaneously expressing EGFR and CD133. Materials & methods: The antitumor activity and mechanism of CESN were investigated. Results & conclusion: The cytotoxicity of CESN in HCC cells and CD133(+) HCC cells was superior to that of A15 or CL4-conjugted or nontargeted salinomycin-loaded nanoparticles. The antitumor assay in mice bearing HCC xenograft tumors confirmed the superior antitumor activity of CESN over other controls. We speculated that the improved therapeutic effect of CESN may be attributed to both targeting a higher percentage of HCC cells and increased delivery of salinomycin to HCC cells.
引用
收藏
页码:1863 / 1879
页数:17
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