Kinetic studies of the interaction between antitumor antibiotics and DNA using quartz crystal microbalance

被引：9

作者：

Tian, L ^{[1
]}

Wei, WZ ^{[1
]}

Mao, Y ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China

来源：

CLINICAL BIOCHEMISTRY | 2004年 / 37卷 / 02期

基金：

中国国家自然科学基金;

关键词：

mitomycin C; bleomycin; DNA; piezoelectric quartz crystal impedance; kinetics;

D O I：

10.1016/j.clinbiochem.2003.10.011

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objective: Kinetic studies of the interaction process between antitumor antibiotics, Mitomycin C (MMC) and Bleomycin (BLM), and DNA were performed with a novel analytical method, piezoelectric quartz crystal (PQC) impedance analysis. Design and methods: DNA was directly immobilized on the Au-electrode surface of a piezoelectric quartz crystal by adsorption. The DNA-modified piezoelectric sensor was in contact with Mitomycin C and Bleomycin solution, respectively. Results: The experimental results demonstrated that antitumor antibiotics concentration had an effect on the interaction. A pseudo-first-order kinetic model for the interaction between antitumor antibiotics and DNA was derived to describe the process. All fitted results were well in agreement with the corresponding experimental results. The kinetic parameters, the interaction rate constants (k(MMC) and k(BLM)), were determined by fitting experimental data to the model. At 37 +/- 0.5degreesC, the k(MMC) and k(BLM) values obtained were 4.56 (+/-0.02) x 10(-3) and 9.11 (+/-0.02) mM(-1) s(-1), respectively. Conclusion: Piezoelectric quartz crystal impedance (PQCI) analysis is a very powerful method to study the kinetic process of antitumor drugs and DNA interaction. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.

引用

页码：120 / 127

页数：8

共 48 条

[1] ANALOG VERSUS DIGITAL RECOGNITION OF DNA BY BLEOMYCIN - AN EFFECT OF THE CARBOHYDRATE MOIETY [J].