Quantitative determination of naphthoquinones of Impatiens species

被引:51
作者
Lobstein, A
Brenne, X
Feist, E
Metz, N
Weniger, B
Anton, R
机构
[1] Univ Strasbourg 1, Fac Pharm Strasbourg, Dept Pharmacognosy, F-67401 Illkirch, France
[2] Univ Strasbourg 1, Dept Med & Cosmet Anal, F-67401 Illkirch, France
关键词
quantitative determination; RP-HPLC; lawsone; 2-hydroxy-1,4-naphthoquinone; 2-methoxy-1,4-naphthoquinone; Impatiens; Balsaminaceae;
D O I
10.1002/pca.574
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A convenient and reliable reversed phase-HPLC method has been developed and validated for the quantitative determination of naphthoquinones present in the aerial parts of Impatiens glandulifera (Balsaminaceae) during two growing seasons (May to August 1998 and June to October 1999). Maximal content (0.8-1.1%) of 2-hydroxy-1,4-naphthoquinone and its 2-methylated derivative was observed in flowers collected between July and August. The procedure was applied to compare the pigment content in three other species of Impatiens but showed 2.5-37 times lower levels than those found in I. glandulifera. Copyright (C) 2001 John Wiley & Sons, Ltd.
引用
收藏
页码:202 / 205
页数:4
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