Target screening and confirmation of 35 licit and illicit drugs and metabolites in hair by LC-MSMS

被引:107
作者
Lendoiro, Elena [1 ]
Quintela, Oscar [2 ]
de Castro, Ana [1 ,3 ]
Cruz, Angelines [1 ]
Lopez-Rivadulla, Manuel [1 ]
Concheiro, Marta [1 ]
机构
[1] Univ Santiago de Compostela, Inst Ciencias Forenses, Serv Toxicol Forense, Santiago De Compostela 15782, A Coruna, Spain
[2] Inst Nacl Toxicol & Ciencias Forenses, Madrid, Spain
[3] Cienytech SL, Dept I D, Santiago De Compostela, Spain
关键词
Hair; Screening; LC-MSMS; THC; TANDEM MASS-SPECTROMETRY; QUANTIFICATION; BENZODIAZEPINES; AMPHETAMINES; DIAZEPAM; OPIATES; COCAINE; ABUSE;
D O I
10.1016/j.forsciint.2011.11.006
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
A liquid chromatography-tandem mass spectrometry (LC-MSMS) target screening in 50 mg hair was developed and fully validated for 35 analytes (Delta 9-tetrahidrocannabinol (THC), morphine, 6-acetylmorphine, codeine, methadone, fentanyl, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, benzoylecgonine, cocaine, lysergic acid diethylamide, ketamine, scopolamine, alprazolam, bromazepam, clonazepam, diazepam, flunitrazepam, 7-aminoflunitrazepam, lorazepam, lormetazepam, nordiazepam, oxazepam, tetrazepam, triazolam, zolpidem, zopiclone, amitriptyline, citalopram, clomipramine, fluoxetine, paroxetine and venlafaxine). Hair decontamination was performed with dichloromethane, and incubation in 2 mL of acetonitrile at 50 degrees C overnight. Extraction procedure was performed in 2 steps, first liquid-liquid extraction, hexane: ethyl acetate (55: 45, v: v) at pH 9, followed by solid-phase extraction (Strata-X cartridges). Chromatographic separation was performed in AtlantisT3 (2.1 mm x 100 mm, 3 mu m) column, acetonitrile and ammonium formate pH 3 as mobile phase, and 32 min total run time. One transition per analyte was monitored in MRM mode. To confirm a positive result, a second injection monitoring 2 transitions was performed. The method was specific (no endogenous interferences, n = 9); LOD was 0.2-50 pg/mg and LOQ 0.5-100 pg/mg; linearity ranged from 0.5-100 to 2000-20,000 pg/mg; imprecision <15%; analytical recovery 85-115%; extraction efficiency 4.1-85.6%; and process efficiency 2.5-207.7%; 27 analytes showed ion suppression (up to -86.2%), 4 ion enhancement (up to 647.1%), and 4 no matrix effect; compounds showed good stability 24-48 h in autosampler. The method was applied to 17 forensic cases. In conclusion, a sensitive and specific target screening of 35 analytes in 50 mg hair, including drugs of abuse (THC, cocaine, opiates, amphetamines) and medicines (benzodiazepines, antidepressants) was developed and validated, achieving lower cut-offs than Society of Hair Testing recommendations. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:207 / 215
页数:9
相关论文
共 22 条
[1]   Guidelines for European workplace drug and alcohol testing in hair [J].
Agius, Ronald ;
Kintz, Pascal .
DRUG TESTING AND ANALYSIS, 2010, 2 (7-8) :367-376
[2]  
[Anonymous], 2010, WORLD DRUG REP 2010
[3]   Quantification of drugs of abuse and some stimulants in hair samples by liquid chromatography-electrospray ionization ion trap mass spectrometry [J].
Bucelli, Francesca ;
Fratini, Angela ;
Bavazzano, Paolo ;
Comodo, Nicola .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (31) :3931-3936
[4]   Simultaneous quantification of opiates, amphetamines, cocaine and metabolites and diazepam and metabolite in a single hair sample using GC-MS [J].
Cordero, Rosa ;
Paterson, Sue .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 850 (1-2) :423-431
[5]  
European Commission, 2002, 2002657EC
[6]   Analysis of ketamine and norketamine in hair samples using molecularly imprinted solid-phase extraction (MISPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [J].
Harun, Norlida ;
Anderson, Robert A. ;
Cormack, Peter A. G. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (07) :2449-2459
[7]   Drug screening of hair by liquid chromatography-tandem mass spectrometry [J].
Hegstad, S. ;
Khiabani, H. Z. ;
Kristoffersen, L. ;
Kunoe, N. ;
Lobmaier, P. P. ;
Christophersen, A. S. .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2008, 32 (05) :364-372
[8]   Testing for atropine and scopolamine in hair by LC-MS-MS after Datura inoxia abuse [J].
Kintz, Pascal ;
Villain, Marion ;
Barguil, Yann ;
Charlot, Jean-Yves ;
Cirimele, Vincent .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2006, 30 (07) :454-457
[9]   Screening for drugs of abuse in hair with ion spray LC-MS-MS [J].
Kronstrand, R ;
Nyström, I ;
Strandberg, J ;
Druid, H .
FORENSIC SCIENCE INTERNATIONAL, 2004, 145 (2-3) :183-190
[10]   Hair analysis for drugs in driver's license regranting. A Swedish pilot study [J].
Kronstrand, Robert ;
Nystrom, Ingrid ;
Forsman, Malin ;
Kall, Kerstin .
FORENSIC SCIENCE INTERNATIONAL, 2010, 196 (1-3) :55-58