Protective effects of proanthocyanidins against cadmium-induced testicular injury through the modification of Nrf2-Keap1 signal path in rats

被引：51

作者：

He, Liu ^{[1
]}

Li, Peng ^{[1
]}

Yu, Li-Hui ^{[1
]}

Li, Lin ^{[1
]}

Zhang, Yi ^{[1
]}

Guo, Yang ^{[1
]}

Long, Miao ^{[1
]}

He, Jian-Bin ^{[1
]}

Yang, Shu-Hua ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Anim Sci & Vet Med, Key Lab Zoonosis Liaoning Prov, Shenyang 110866, Liaoning, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2018年 / 57卷

基金：

中国国家自然科学基金;

关键词：

Proanthocyanidins; Cadium; Oxidative damage; Nrf2-Keap1; Signal path; Testes; Wistar rats; GRAPE SEED PROANTHOCYANIDINS; OXIDATIVE STRESS; ANTIOXIDANT ACTIVITY; EXTRACT; ACCUMULATION; MECHANISMS; TOXICITY; EXPOSURE; DAMAGE; DNA;

D O I：

10.1016/j.etap.2017.11.002

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The purpose of this study was to evaluate the potential chemoprotective effects of proanthocyanidins (PAs) against cadmium (Cd)-induced oxidative damage of testes via Nrf2-Keap1 signal pathway in rats. Briefly, by using biochemical histological analysis, as well as the real time PCR and western blot approach, oxidative damage in rat testicular tissue was observed after exposure to Cd. In addition, significant down-regulations of mRNA and protein levels of nuclear erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1), gamma-glutamyl cysteine synthetase (gamma-GCS), glutathione peroxidase (GSH-Px) and quinone oxidoreductase 1 (NQO1), as well as a significant up-regulation of Kelch sample related protein-1 (Keap1) levels in testicular tissue were observed after Cd exposure. Notably, these alterations were reverted back to near normalcy in Cd + PAs group rats. In conclusion, PAs exhibited a significant chemopreventive potential against Cd-induced testicular oxidative damage in rats, possibly through the modification of Nrf2-Keap1 signal path.

引用

页码：1 / 8

页数：8

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