New cytotoxic 4-alkyl-dihydroxyfuran coumarins from Mesua ferrea

Four new 4-alkyl-dihydroxyfuran coumarins, Mesuaferols D-F and iso-Mesuaferol F (1- 4 ) were isolated from the flowering buds of Mesua ferrea . The chemical structures of these compounds were confirmed by the extensive analyses of NMR, UV, IR and HR-ESI-MS methods, the absolute configurations were established on the basis of the Mosher's method, electron circular dichroism (ECD) measurement and theoretical calculation methods. The inhibitory activities of the isolates were evaluated on MDA-MB-231, MCF-7 and HepG2 cancer cell lines. All compound s exhibited potent activity against MCF-7 with IC50 as 26.68 mu M, 37.75 mu M, 20.53 mu M and 33.43 mu M respectively, while almost all compound s showed moderate cytotoxic activities against MDA-MB-231 and HepG2 cell lines. The results of prediction potential targets and molecule docking revealed that Mesuaferol D showed the most binding potential against mTOR.