Two Years of Genomic Surveillance in Belgium during the SARS-CoV-2 Pandemic to Attain Country-Wide Coverage and Monitor the Introduction and Spread of Emerging Variants

被引：13

作者：

Cuypers, Lize ^{[1
]}

Dellicour, Simon ^{[2
,3
]}

Hong, Samuel L. ^{[3
]}

Potter, Barney, I ^{[3
]}

Verhasselt, Bruno ^{[4
]}

Vereecke, Nick ^{[5
]}

Lambrechts, Laurens ^{[6
,7
]}

Durkin, Keith ^{[8
]}

Bours, Vincent ^{[8
,9
]}

Klamer, Sofieke ^{[10
]}

Bayon-Vicente, Guillaume ^{[11
]}

Vael, Carl ^{[12
]}

Arien, Kevin K. ^{[13
,14
]}

De Mendonca, Ricardo ^{[15
]}

Soetens, Oriane ^{[16
]}

Michel, Charlotte ^{[17
]}

Bearzatto, Bertrand ^{[18
]}

Naesens, Reinout ^{[19
]}

Gras, Jeremie ^{[20
]}

Vankeerberghen, Anne ^{[21
]}

Matheeussen, Veerle ^{[22
]}

Martens, Geert ^{[23
]}

Obbels, Dagmar ^{[24
]}

Lemmens, Ann ^{[25
]}

Van den Poel, Bea ^{[26
]}

Van Even, Ellen ^{[27
]}

De Rauw, Klara ^{[28
]}

Waumans, Luc ^{[29
]}

Reynders, Marijke ^{[30
]}

Degosserie, Jonathan ^{[31
,32
]}

Maes, Piet ^{[3
]}

Andre, Emmanuel ^{[1
,33
,34
]}

Baele, Guy ^{[3
]}

机构：

[1] Univ Hosp Leuven, Natl Reference Ctr Resp Pathogens, Dept Lab Med, B-3000 Leuven, Belgium

[2] Univ Libre Bruxelles, Spatial Epidemiol Lab SpELL, B-1000 Brussels, Belgium

[3] Katholieke Univ Leuven, Dept Microbiol Immunol & Transplantat, Rega Inst, B-3000 Leuven, Belgium

[4] Univ Ghent, Ghent Univ Hosp, Dept Diagnost Sci, B-9000 Ghent, Belgium

[5] Univ Ghent, Fac Vet Med, PathoSense BV, B-9820 Merelbeke, Belgium

[6] Univ Ghent, Ghent Univ Hosp, HIV Cure Res Ctr, Dept Internal Med & Pediat, B-9000 Ghent, Belgium

[7] Univ Ghent, Fac Biosci Engn, Dept Data Anal & Math Modelling, BioBix, B-9000 Ghent, Belgium

[8] GIGA Res Inst, Lab Human Genet, B-4000 Liege, Belgium

[9] Univ Hosp Liege, Dept Human Genet, B-4000 Liege, Belgium

[10] Sciensano, Sci Directorate Epidemiol & Publ Hlth, B-1050 Brussels, Belgium

[11] Univ Mons, Res Inst Biosci, Dept Prote & Microbiol, B-7000 Mons, Belgium

[12] AZ Klina, Clin Lab, B-2930 Brasschaat, Belgium

[13] Inst Trop Med Antwerp, Dept Biomed Sci, Virol Unit, B-2000 Antwerp, Belgium

[14] Univ Antwerp, Fac Pharmaceut Biomed & Vet Sci, Dept Biomed Sci, B-2000 Antwerp, Belgium

[15] Univ Libre Bruxelles, Dept Microbiol, CUB Hop Erasme, B-1000 Brussels, Belgium

[16] Vrije Univ Brussel, Univ Ziekenhuis Brussel, Dept Microbiol & Infect Control, B-1090 Brussels, Belgium

[17] Lab Hosp Univ Bruxelles LHUB ULB, Dept Microbiol, B-1000 Brussels, Belgium

[18] Univ Catholique Louvain UCLouvain, Ctr Appl Mol Technol CTMA, Inst Rech Expt & Clin IREC, B-1000 Brussels, Belgium

[19] Ziekenhuis Netwerk Antwerpen, Dept Med Microbiol, B-2020 Antwerp, Belgium

[20] Inst Pathol & Genet IPG, B-6041 Gosselies, Belgium

[21] Onze Lieve Vrouw Hosp, Lab Mol Biol, Campus Aalst Asse Ninove, B-9300 Aalst, Belgium

[22] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, Dept Microbiol, Lab Med Microbiol, B-2610 Antwerp, Belgium

[23] AZ Delta Gen Hosp, Dept Lab Med, B-8800 Roeselare, Belgium

[24] Imelda Hosp, Clin Lab, B-2820 Bonheiden, Belgium

[25] AZ Sint Maarten Hosp, Lab Clin Biol, B-2800 Mechelen, Belgium

[26] Gen Hosp Jan Portaels, Clin Lab, B-1800 Vilvoorde, Belgium

[27] HH Hosp Lier, Clin Lab Microbiol, B-2500 Lier, Belgium

[28] AZ Sint Lucas Hosp, Lab Clin Biol, B-9000 Ghent, Belgium

[29] Jessa Hosp, Clin Lab, B-3500 Hasselt, Belgium

[30] AZ Sint Jan Bruges Ostend AV, Dept Lab Med, Med Microbiol, B-8000 Brugge, Belgium

[31] CHU UCL Namur, Dept Lab Med, Fed Testing Platform COVID 19, B-5530 Yvoir, Belgium

[32] CHU UCL Namur, Mol Diagnost Ctr, Next Generat Sequencing Platform, B-5530 Yvoir, Belgium

[33] Univ Hosp Leuven, Dept Lab Med, Fed Testing Platform COVID 19, B-3000 Leuven, Belgium

[34] Katholieke Univ Leuven, Lab Clin Microbiol, Dept Microbiol Immunol & Transplantat, B-3000 Leuven, Belgium

来源：

VIRUSES-BASEL | 2022年 / 14卷 / 10期

基金：

欧盟地平线“2020”;

关键词：

SARS-CoV-2; COVID-19; Belgium; genomic surveillance; next-generation sequencing; variants of concern;

D O I：

10.3390/v14102301

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An adequate SARS-CoV-2 genomic surveillance strategy has proven to be essential for countries to obtain a thorough understanding of the variants and lineages being imported and successfully established within their borders. During 2020, genomic surveillance in Belgium was not structurally implemented but performed by individual research laboratories that had to acquire the necessary funds themselves to perform this important task. At the start of 2021, a nationwide genomic surveillance consortium was established in Belgium to markedly increase the country's genomic sequencing efforts (both in terms of intensity and representativeness), to perform quality control among participating laboratories, and to enable coordination and collaboration of research projects and publications. We here discuss the genomic surveillance efforts in Belgium before and after the establishment of its genomic sequencing consortium, provide an overview of the specifics of the consortium, and explore more details regarding the scientific studies that have been published as a result of the increased number of Belgian SARS-CoV-2 genomes that have become available.

引用

页数：19

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