Separation and Purification of Gardecin from Gardenia jasminoides Ellis by High-Speed Countercurrent Chromatography

被引：4

作者：

Chen, Yang ^{[1
]}

Deng, Liang ^{[2
,3
]}

Wang, Wenjun ^{[1
]}

Xia, Qiang ^{[1
]}

Zhou, Xumei ^{[4
]}

Wang, Yarong ^{[1
]}

Hou, Huijing ^{[1
]}

Cai, Shining ^{[1
]}

Cui, Guozhen ^{[1
]}

Chen, Ping ^{[5
]}

Lu, Tao ^{[5
]}

Zhang, Hao ^{[5
]}

机构：

[1] Zunyi Med Coll, Dept Pharmaceut Sci, Zhuhai 51904, Guangdong, Peoples R China

[2] Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China

[3] Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China

[4] Zunyi Med Univ, Dept Pharmaceut Anal, Zunyi, Guizhou, Peoples R China

[5] Sichuan Univ, West China Sch Pharm, Chengdu 610064, Sichuan, Peoples R China

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2015年 / 50卷 / 12期

关键词：

gardecin; separation and purification; crocetin derivative (crocin); high-speed counter-current chromatography (HSCCC); antioxidant activity; Gardenia jasminoides Ellis; PREPARATIVE ISOLATION; ANTIOXIDANT ACTIVITY; CROCIN;

D O I：

10.1080/01496395.2015.1013636

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the present study, a high-speed counter-current chromatography (HSCCC) was investigated for separation and purification of gardecin on a preparative scale. Hexane-ethyl acetate-methanol-water (0.3:1:0.3:1, v/v) was selected as the optimum solvent system to purify gardecin from a fraction obtained from HPD100 column chromatography fractionation of gardenia. After HSCCC isolation, 20.1 mg of gardecin with purity of 97.4% was obtained from 322 g of dry gardenia fruits. Chemical structure identification of this pigment was carried out by MS, H-1 NMR, and C-13 NMR. Additionally, antioxidant activity of gardecin in comparison with crocin-1 was investigated. The present results demonstrated that gardecin could be efficiently obtained using HSCCC from this herb and this compound features strong antioxidant activity.

引用

页码：1899 / 1905

页数：7

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