Effects of positively selected sequence variations in human and Macaca fascicularis β-defensins 2 on antimicrobial activity

被引：45
作者：
Antcheva, N
Boniotto, M
Zelezetsky, I
Pacor, S
Falzacappa, MVV
Crovella, S
Tossi, A
机构：
[1] Univ Trieste, Dept Biochem Biophys & Macromol Chem, I-34127 Trieste, Italy
[2] Univ Trieste, Dept Reprod & Dev Sci, I-34127 Trieste, Italy
[3] Univ Trieste, Dept Biomed Sci, I-34127 Trieste, Italy
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 02期
关键词：
D O I：
10.1128/AAC.48.2.685-688.2004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The evolution of orthologous genes coding for beta-defensin 2 (BD2) in primates has been subject to positive selection during the divergence of the platyrrhines from the catarrhines and of the Cercopithecidae from the Hylobatidae, great apes, and humans. Three peptides have been selected for a functional analysis of the effects of sequence variations on the direct antimicrobial activity: human BD2 (hBD2), Macaca fascicularis BD2 (mfaBD2), and a variant of the human peptide lacking Asp(4), (-D)hBD2, which is characteristic only of the human/great ape peptides. hBD2 and mfaBD2 showed a significant difference in specificity, the former being more active towards Escherichia coli and the later towards Staphylococcus aureus and Candida albicans. Asp(4) in the human peptide appears to be important, as (-D)hBD2 was less structured and had a markedly lower antimicrobial activity. The evolution of beta-defensin 2 in primates may thus have been driven, at least in part, by different environmental pressures so as to modulate antimicrobial activity.
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收藏
页码：685 / 688
页数：4
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