Investigation of the virulence and genomics of Aeromonas salmonicida strains isolated from human patients

被引：40

作者：

Vincent, Antony T. ^{[1
]}

Fernandez-Bravo, Ana ^{[2
]}

Sanchis, Marta ^{[2
]}

Mayayo, Emilio ^{[2
,3
]}

Jose Figueras, Maria ^{[2
]}

Charette, Steve J. ^{[1
]}

机构：

[1] Univ Laval, Quebec City, PQ, Canada

[2] Univ Rovira & Virgili, IISPV, Reus, Spain

[3] Univ Hosp Joan XIII, Tarragona, Spain

来源：

INFECTION GENETICS AND EVOLUTION | 2019年 / 68卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Aeromonas salmonicida; Infection; Necrotizing fasciitis; Pathogenicity; Type III secretion systems; Whole genome sequencing; III SECRETION SYSTEM; RESISTANCE; IDENTIFICATION; TEMPERATURE; GENES;

D O I：

10.1016/j.meegid.2018.11.019

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The bacterium Aeromonas salmonicida is known since long time as a major fish pathogen unable to grow at 37 degrees C. However, some cases of human infection by putative mesophilic A. salmonicida have been reported. The goal of the present study is to examine two clinical cases of human infection by A. salmonicida in Spain and to investigate the pathogenicity in mammals of selected mesophilic A. salmonicida strains. An evaluation of the pathogenicity in a mouse model of clinical and environmental A. salmonicida strains was performed. The genomes of the strains were sequenced and analyzed in order to find the virulence determinants of these strains. The experimental infection in mice showed a gradient in the virulence of these strains and that some of them can cause necrotizing fasciitis and tissue damage in the liver. In addition to demonstrating significant genomic diversity among the strains studied, bioinformatics analyses permitted also to shed light on crucial elements for the virulence of the strains, like the presence of a type III secretion system in the one that caused the highest mortality in the experimental infection. Clinicians and microbiologists should consider these results for the inclusion of A. salmonicida in diagnosis tests since it is now clear that some mesophilic strains are also pathogens for humans.

引用

页码：1 / 9

页数：9

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