Peroxisome proliferator-activated receptor γ induces growth arrest and differentiation markers of human colon cancer cells

被引：249

作者：

Kitamura, S ^{[1
]}

Miyazaki, Y ^{[1
]}

Shinomura, K ^{[1
]}

Kondo, S ^{[1
]}

Kanayama, S ^{[1
]}

Matsuzawa, Y ^{[1
]}

机构：

[1] Osaka Univ, Sch Med, Dept Internal Med 2, Osaka 5650871, Japan

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1999年 / 90卷 / 01期

关键词：

PPAR gamma; colon cancer cells; differentiation;

D O I：

10.1111/j.1349-7006.1999.tb00668.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Peroxisome proliferator-activated receptor gamma (PPAR gamma), one of the nuclear receptors expressed in adipose tissue, plays an important role in adipocyte differentiation. In this study, we investigated the expression of PPAR gamma and its role in cellular growth and differentiation in six colon cancer cell lines: HT-29, CaCo-2, SW-480, DLD-1, LoVo, and T-84. All sis expressed PPAR gamma mRNA and protein, shown respectively on northern and western blot analyses. Luciferase assay in HT-29 cells, which strongly express PPAR gamma, showed that troglitazone, a selective ligand for PPAR gamma, transactivated the transcription of a peroxisome proliferator response element (PPRE)-driven promoter. Furthermore, troglitazone caused a marked decrease in [H-3]thymidine incorporation and G1 cell-cycle arrest determined by flow cytometry. Finally, troglitazone induced expression of mRNAs for villin and intestinal alkaline phosphatase, markers for enterocyte differentiation. In conclusion, human colon cancer cells express PPAR gamma the ligands of which inhibit cell growth and induce differentiation markers.

引用

页码：75 / 80

页数：6

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