UDP-N-acetylglucosamine 4-epimerase activity indicates the presence of N-acetylgalactosamine in exopolysaccharides of Streptococcus thermophilus strains

被引:26
作者
Degeest, B
Vaningelgem, F
Laws, AP
De Vuyst, L
机构
[1] Free Univ Brussels, Res Grp Ind Microbiol Fermentat Technol & Downstr, Dept Appl Biol Sci, B-1050 Brussels, Belgium
[2] Univ Huddersfield, Sch Appl Sci, Huddersfield HD1 3DH, W Yorkshire, England
关键词
D O I
10.1128/AEM.67.9.3976-3984.2001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The monomer composition of the exopolysaccharides (EPS) produced by Streptococcus thermophilus LY03 and S. thermophilus Sfi20 were evaluated by high-pressure liquid chromatography with amperometric detection and nuclear magnetic resonance spectroscopy. Both strains produced the same EPS composed of galactose, glucose, and N-acetylgalactosamine. Further, it was demonstrated that the activity of the precursor-producing enzyme UDP-N-acetylglucosamine 4-epimerase, converting UDP-N-acetylglucosamine into UDP-N-acetylgalactosamine, is responsible for the presence of N-acetylgalactosamine in the EPS repeating units of both strains. The activity of UDP-N-acetylglucosamine 4-epimerase was higher in both S. thermophilus strains than in a non-EPS-producing control strain. However, the level of this activity was not correlated with EPS yields, a result independent of the carbohydrate source applied in the fermentation process. On the other hand, both the amounts of EPS and the carbohydrate consumption rates were influenced by the type of carbohydrate source used during S. thermophilus Sfi20 fermentations. A correlation between activities of the enzymes alpha -phosphoglucomutase, UDP-glucose pyrophosphorylase, and UDP-galactose 4-epimerase and EPS yields was seen. These experiments confirm earlier observed results for S. thermophilus LY03, although S. thermophilus Sfi20 preferentially consumed glucose for EPS production instead of lactose in contrast to the former strain.
引用
收藏
页码:3976 / 3984
页数:9
相关论文
共 50 条
  • [1] The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide
    Almirón-Roig, E
    Mulholland, F
    Gasson, MJ
    Griffin, AM
    [J]. MICROBIOLOGY-SGM, 2000, 146 : 2793 - 2802
  • [2] Are horizontal transfers involved in the evolution of the Streptococcus thermophilus exopolysaccharide synthesis loci?
    Bourgoin, F
    Pluvinet, A
    Gintz, B
    Decaris, B
    Guédon, G
    [J]. GENE, 1999, 233 (1-2) : 151 - 161
  • [3] Structural characterisation of the exocellular polysaccharide produced by Streptococcus thermophilus OR 901
    Bubb, WA
    Urashima, T
    Fujiwara, R
    Shinnai, T
    Ariga, H
    [J]. CARBOHYDRATE RESEARCH, 1997, 301 (1-2) : 41 - 50
  • [4] Cerning J., 1999, Recent Research Developments in Microbiology, V3, P195
  • [5] DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x
  • [6] De Vuyst L, 1998, J APPL MICROBIOL, V84, P1059
  • [7] Heteropolysaccharides from lactic acid bacteria
    De Vuyst, L
    Degeest, B
    [J]. FEMS MICROBIOLOGY REVIEWS, 1999, 23 (02) : 153 - 177
  • [8] Degeest B, 1999, APPL ENVIRON MICROB, V65, P2863
  • [9] Correlation of activities of the enzymes α-phosphoglucomutase, UDP-galactose 4-epimerase, and UDP-glucose pyrophosphorylase with exopolysaccharide biosynthesis by Streptococcus thermophilus LY03
    Degeest, B
    De Vuyst, L
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) : 3519 - 3527
  • [10] DEGEEST B, IN PRESS J APPL MICR