A Class of Human Proteins that Deliver Functional Proteins into Mammalian Cells In Vitro and In Vivo

被引：92

作者：

Cronican, James J. ^{[1
]}

Beier, Kevin T. ^{[2
,3
]}

Davis, Tina N. ^{[4
,5
,6
]}

Tseng, Jen-Chieh ^{[4
,5
,6
]}

Li, Weida ^{[7
]}

Thompson, David B. ^{[1
]}

Shih, Allen F. ^{[1
]}

May, Erin M. ^{[1
]}

Cepko, Constance L. ^{[2
,3
]}

Kung, Andrew L. ^{[4
,5
,6
]}

Zhou, Qiao ^{[7
]}

Liu, David R. ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[2] Harvard Univ, Sch Med, Dept Genet, Howard Hughes Med Inst, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Lurie Family Imaging Ctr, Boston, MA 02115 USA

[5] Harvard Univ, Sch Med, Dept Pediat Oncol, Dana Farber Canc Inst, Boston, MA 02115 USA

[6] Harvard Univ, Sch Med, Childrens Hosp, Boston, MA 02115 USA

[7] Harvard Univ, Harvard Stem Cell Inst, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

来源：

CHEMISTRY & BIOLOGY | 2011年 / 18卷 / 07期

基金：

美国国家卫生研究院;

关键词：

HUMAN IMMUNODEFICIENCY VIRUS; MEMBRANE-PERMEABLE PEPTIDES; PENETRATING PEPTIDES; FUSION PROTEIN; DEK; DNA;

D O I：

10.1016/j.chembiol.2011.07.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We discovered a class of naturally occurring human proteins with unusually high net positive charge that can potently deliver proteins in functional form into mammalian cells both in vitro and also in murine retina, pancreas, and white adipose tissues in vivo. These findings represent diverse macromolecule delivery agents for in vivo applications, and also raise the possibility that some of these human proteins may penetrate cells as part of their native biological functions.

引用

页码：833 / 838

页数：6

共 32 条

[1] Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences [J].

Atkinson, Helen ;

Chalmers, Ronald .

GENETICA, 2010, 138 (05) :485-498

[2] The transcription factor Engrailed-2 guides retinal axons [J].

Brunet, I ;

Weinl, C ;

Piper, M ;

Trembleau, A ;

Volovitch, M ;

Harris, W ;

Prochiantz, A ;

Holt, C .

NATURE, 2005, 438 (7064) :94-98

[3] Intracellular delivery of a Tat-eGFP fusion protein into muscle cells [J].

Caron, NJ ;

Torrente, Y ;

Camirand, G ;

Bujold, M ;

Chapdelaine, P ;

Leriche, K ;

Bresolin, N ;

Tremblay, JP .

MOLECULAR THERAPY, 2001, 3 (03) :310-318

[4]

Chandler LA, 1998, INT J CANCER, V78, P106, DOI 10.1002/(SICI)1097-0215(19980925)78:1<106::AID-IJC17>3.0.CO

[5]

2-9

[6] HDAC4 Regulates Neuronal Survival in Normal and Diseased Retinas [J].

Chen, Bo ;

Cepko, Constance L. .

SCIENCE, 2009, 323 (5911) :256-259

[7] Hepatocyte growth factor (HGF)/NK1 is a naturally occurring HGF scatter factor variant with partial agonist antagonist activity [J].

Cioce, V ;

Csaky, KG ;

Chan, AML ;

Bottaro, DP ;

Taylor, WG ;

Jensen, R ;

Aaronson, SA ;

Rubin, JS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (22) :13110-13115

[8] Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged Protein [J].

Cronican, James J. ;

Thompson, David B. ;

Beier, Kevin T. ;

McNaughton, Brian R. ;

Cepko, Constance L. ;

Liu, David R. .

ACS CHEMICAL BIOLOGY, 2010, 5 (08) :747-752

[9] Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation [J].

Elenius, K ;

Paul, S ;

Allison, G ;

Sun, J ;

Klagsbrun, M .

EMBO JOURNAL, 1997, 16 (06) :1268-1278

[10] CELLULAR UPTAKE OF THE TAT PROTEIN FROM HUMAN IMMUNODEFICIENCY VIRUS [J].

FRANKEL, AD ;

PABO, CO .

CELL, 1988, 55 (06) :1189-1193

← 1 2 3 4 →