Electrochemical genosensors as innovative tools for detection of genetically modified organisms

被引：58

作者：

Lorena Manzanares-Palenzuela, C. ^{[1
]}

Martin-Fernandez, Begona ^{[1
]}

Sanchez-Paniagua Lopez, Marta ^{[1
]}

Lopez-Ruiz, Beatriz ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Farm, Secc Dept Quim Analit, E-28040 Madrid, Spain

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2015年 / 66卷

关键词：

Detection method; DNA detection; Electrochemical DNA biosensor; Electrochemical genosensor; Food product; Genetically modified organism; GMO; Selectivity; Transgenic trait; WALLED CARBON NANOTUBES; GENE SEQUENCE DETECTION; DNA HYBRIDIZATION; PAT GENE; MODIFIED MAIZE; SULFIDE NANOPARTICLES; DEOXYRIBONUCLEIC-ACID; SENSITIVE DETECTION; PCR AMPLIFICATION; GOLD ELECTRODES;

D O I：

10.1016/j.trac.2014.10.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A genetically modified organism (GMO) is defined as a living organism whose genome has been modified by the introduction of an exogenous gene able to express an additional protein that confers new characteristics, such as enhancement of the nutritional properties, herbicide resistance or insect protection. The need to monitor and to verify the presence and the amount of GMOs in agricultural crops and in food products has generated interest in analytical methods for accurate, sensitive, rapid, cheap detection of these products. A novel DNA-detection technology was developed: genosensors. This article reviews electrochemical DNA biosensors reported for the qualitative and quantitative determination of transgenic traits. We discuss critical aspects of genosensor design with particular emphasis on analytical characteristics and analysis of real samples. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：19 / 31

页数：13

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