Potential role of heat-shock protein 70 and interleukin-15 in the pathogenesis of threatened spontaneous abortions

被引：14

作者：

Gulic, Tamara ^{[1
]}

Laskarin, Gordana ^{[1
,2
]}

Dominovic, Marin ^{[1
]}

Gacanin, Lana Glavan ^{[3
]}

Babarovic, Emina ^{[4
]}

Haller, Herman ^{[3
]}

Rukavina, Daniel ^{[1
,5
]}

机构：

[1] Univ Rijeka, Dept Physiol & Immunol, Fac Med, Rijeka, Croatia

[2] Hearth & Lung Dis & Rheumatism Thalassotherapia O, Hosp Med Rehabil, Div Cardiol, Opatija, Croatia

[3] Univ Rijeka, Clin Hosp, Dept Obstet & Gynecol, Rijeka, Croatia

[4] Univ Rijeka, Fac Med, Dept Pathol, Rijeka, Croatia

[5] Croatian Acad Sci & Arts, Dept Clin & Transplantat Immunol & Mol Med Rijeka, Rijeka, Croatia

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2016年 / 76卷 / 02期

关键词：

granulysin; heat-shock protein 70; interleukin-15; pathological pregnancy; perforin; NATURAL-KILLER-CELLS; HEAT-SHOCK PROTEINS; MATERNAL-FETAL INTERFACE; DENDRITIC CELLS; INTRACELLULAR TRAFFICKING; RECURRENT MISCARRIAGE; PERFORIN EXPRESSION; MISSED ABORTION; NK CELLS; PREGNANCY;

D O I：

10.1111/aji.12525

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ProblemThe role of HSP70 and both its constitutive (Hsc) and inducible (Hsp) forms in the pathogenesis of threatened spontaneous abortions was investigated. Method of studyImmunohistology and/or immunofluorescence was used to analyze paraffin-embedded tissue sections, and reverse transcriptase-quantitative polymerase chain reaction and flow cytometry were used for analyses of decidual mononuclear cells (DMCs) and confocal microscopy for the detection of perforin, granulysin, and lysosome-associated membrane protein-1 (LAMP-1) in decidual lymphocytes (DLs). ResultsThe percentage of single Hsp70(+), Hsc70(+), and IL-15(+) cells and mRNA levels of HSP70, CD91, and TLR4 were lower in the decidua basalis in cases of threatened miscarriages compared to that in cases of normal pregnancy. In a suspension of normal DMCs, IL-15 significantly decreased the HSP70 members and TLR4 in dendritic cells, T cells, and NK cells while increasing CD91 in NK cells alone. ConclusionDownregulation of Hsc70, Hsp70, and IL-15 expression at gene and/or protein levels might support the retention of fertilization products in cases of missed abortion and blighted ovum.

引用

页码：126 / 136

页数：11