Salt Stress-Induced Anthocyanin Biosynthesis Genes and MATE Transporter Involved in Anthocyanin Accumulation in Daucus carota Cell Culture

被引：14

作者：

Saad, Kirti R. ^{[1
]}

Kumar, Gyanendra ^{[1
]}

Mudliar, Sandeep N. ^{[1
]}

Giridhar, Parvatam ^{[1
]}

Shetty, Nandini P. ^{[1
]}

机构：

[1] CSIR Cent Food Technol Res Inst, Plant Cell Biotechnol Dept, Mysuru 570020, Karnataka, India

来源：

ACS OMEGA | 2021年 / 6卷 / 38期

关键词：

SUSPENSION-CULTURES; BIOACTIVE COMPOUNDS; ADVENTITIOUS ROOTS; CALLUS-CULTURES; SHAKE FLASKS; BIOREACTOR; BIOMASS; OPTIMIZATION; CULTIVATION; ACID;

D O I：

10.1021/acsomega.1c02941

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Anthocyanins biosynthesis is a well-studied biosynthesis pathway in Daucus carota. However, the scale-up production at the bioreactor level and transporter involved in accumulation is poorly understood. To increase anthocyanin content and elucidate the molecular mechanism involved in accumulation, we examined D. carota cell culture in flask and bioreactor for 18 days under salt stress (20.0 mM NH4NO3/37.6 mM KNO3) at 3 day intervals. The expression of anthocyanin biosynthesis and putative MATE (multidrug and toxic compound extrusion) transporter expression was analyzed by qRT-PCR. It was observed that there was a significant enhancement of anthocyanin in the bioreactor compared to the control culture. A correlation was observed between the expression of MATE and the anthocyanin biosynthesis genes (CHS, C4H, LDOX, and UFGT) on the 9th day in a bioreactor, where maximum anthocyanin accumulation and expression was detected. We hypothesize the involvement of MATE in transporting anthocyanin to tonoplast in D. carota culture under salt stress.

引用

页码：24502 / 24514

页数：13

共 47 条

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