Stability of plasma gamma-hydroxybutyrate determined by gas chromatography-positive ion chemical ionization-mass spectrometry

被引:17
作者
Chen, M [1 ]
Andrenyak, DM [1 ]
Moody, DE [1 ]
Foltz, RL [1 ]
机构
[1] Univ Utah, Dept Pharmacol & Toxicol, Ctr Human Toxicol, Salt Lake City, UT 84112 USA
关键词
4-HYDROXYBUTYRIC ACID; BUTYROLACTONE GBL; GHB; URINE; BRAIN; RAT; METABOLISM; BLOOD; DRUG;
D O I
10.1093/jat/27.7.445
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An effective method for the determination of gamma-hydroxybutyric acid (GHB) in human plasma is described that utilizes a simple liquid-liquid extraction procedure and gas chromatography-positive ion chemical ionization-mass spectrometry (GC-PCI-MS). The method has been used to study the stability of plasma GHB under several storage conditions. Following the extraction with acetonitrile, GHB and deuterated GHB (GHB-d6) were derivatized with N,O-bis[trimethylsilyl] trifluoroacetamide (BSFTA). After the separation on a capillary GC column, the derivatives were ionized with ammonia reagent gas and analyzed by MS. The lower limit of quantitation in 100 μL of plasma was 2.5 μg/mL, over a range from 2.5 to 250 μg/mL. The coefficients of variation did not exceed 3.9% and the mean measured concentrations did not deviate more than 8% from the target for both intra- and interassay precision and accuracy. Plasma GHB was found to be stable at -20°C for up to 9 months, at room temperature for 48 h, and after 3 freeze/thaw cycles. It was also found to be stable in processed samples stored at room temperature for 5 days and for 15 days at -20°C.
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页码:445 / 448
页数:4
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