Genetic analysis of antibiotic-resistance determinants in multidrug-resistant Shigella strains isolated from Chilean children

被引：67

作者：

Toro, CS

Farfán, M

Contreras, I

Flores, O

Navarro, N

Mora, GC

Prado, V

机构：

[1] Univ Chile, Fac Med, Inst Ciencias Biomed, Lab Antobiol,Programa Microbiol & Micol, Santiago, Chile

[2] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Bioquim & Biol Mol, Santiago, Chile

[3] Univ Andres Bello, Dept Ciencias Biol, Microbiol Lab, Santiago, Chile

来源：

EPIDEMIOLOGY AND INFECTION | 2005年 / 133卷 / 01期

关键词：

D O I：

10.1017/S0950268804003048

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A total of 162 clinical isolates of Shigella collected from children in a semi-rural community of Chile were examined for the presence of genetic determinants of resistance to ampicillin, chloramphenicol, tetracycline, and trimethoprim. Ampicillin resistance was most frequently associated with the presence of bla(OXA) in S.flexneri and with bla(TEM) in S. sonnei. The bla(OXA) gene but not bla(TEM) was located in class 1 integrons. The dhfrIa gene encoding for resistance to trimethoprim was associated to class 2 integrons and detected exclusively in S.flexneri, whereas dhfrIIIc was found in all S. sonnei strains and in 10 % of the S. I S. flexneri isolates. Cat, coding for choramphenicol resistance, and bla(OXA) genes were located in the chromosome in all cases, whereas tet A gene, coding for tetracycline resistance, and bla(TEM), dhfrIa and dhfrIIIc genes were found either in the chromosome or in conjugative plasmids. Our results show a heterogenous distribution of antibiotic-resistance determinants between S.flexneri and S. sonnei.

引用

页码：81 / 86

页数：6

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