Food-borne Enterococci Integrate Into Oral Biofilm: An In Vivo Study

被引:27
作者
Al-Ahmad, Ali [1 ]
Maier, Julia
Follo, Marie [2 ]
Spitzmueller, Bettina
Wittmer, Annette [3 ]
Hellwig, Elmar
Huebner, Johannes [4 ]
Jonas, Daniel [5 ]
机构
[1] Univ Freiburg, Dept Operat Dent & Periodontol, Univ Sch, D-79106 Freiburg, Germany
[2] Univ Freiburg, Dept Hematol & Oncol, Core Facil, D-79106 Freiburg, Germany
[3] Univ Freiburg, Inst Med Microbiol & Hyg, D-79106 Freiburg, Germany
[4] Univ Hosp Freiburg, Div Infect Dis, Freiburg, Germany
[5] Univ Hosp Freiburg, Dept Environm Hlth Sci, Freiburg, Germany
关键词
Duplex FISH; endodontic infection; enterococci; Enterococcus faecalis; food; oral biofilm; ROOT-FILLED TEETH; VANCOMYCIN-RESISTANT ENTEROCOCCI; PRIMARY ENDODONTIC INFECTIONS; LASER-SCANNING MICROSCOPY; SEQUENCE TYPING SCHEME; SITU HYBRIDIZATION; APICAL PERIODONTITIS; FAECALIS; BACTERIA; IDENTIFICATION;
D O I
10.1016/j.joen.2010.08.011
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction: Enterococci, particulary Enterococcus faecalis, are still a primary concern in endodontic infections. To date, enterococci have been considered to be only transiently present in the oral cavity. The aim of this study was to examine whether different enterococci from food are able to reside in oral biofilm. Methods: Six healthy volunteers wore dental splints loaded with enamel slabs. After 3 days, the volunteers consumed cheese containing enterococci. The fate of the enterococci was analyzed by culture technique and 165 rRNA gene sequencing. All isolates were characterized genotypically by macrorestriction analysis (Smal) and pulsed-field gel electrophoresis. E. faecalis was also analyzed by using fluorescent in situ hybridization (FISH). Results: E. faecalis, E. faecium, E. avium, and E. durans were detected in the initial biofilm after 2 hours, as well as in the 5-day-old oral biofilm. E. faecalis, E. faecium, and E. avium isolated from the initial biofilm and from the 5-day-old biofilm, as well as those isolated from cheese, showed genetic homogeneity. E. faecium and E. avium had integrated into a pre-existing 3-day-old biofilm. No genetic similarity between E. durans strains isolated from cheese and those from the initial and 5-day-old oral biofilm was detected. E. faecalis was also detected in the oral biofilm by using FISH. Conclusions: Food-borne enterococci, particularly E. faecalis, might not only be transient but could also survive in the oral biofilm and become a source for endodontic infections. Moreover, genotypic analysis is required to study the source of oral enterococci. (J Endod 2010;36:1812-1819)
引用
收藏
页码:1812 / 1819
页数:8
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