Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

被引：117

作者：

Joo, JH

Yoo, HJ

Hwang, I

Lee, JS

Nam, KH ^{[1
]}

Bae, YS

机构：

[1] Ewha Womans Univ, Ctr Cell Signaling Res, Div Mol Life Sci, Seoul 120750, South Korea

[2] Ewha Womans Univ, Ctr Cell Signaling Res, Dept Biol Sci, Seoul 120750, South Korea

[3] Pohang Univ Sci & Technol, Ctr Plant Intracellular Trafficking, Pohang 790784, South Korea

[4] Sookmyung Womens Univ, Dept Sci Biol, Seoul 140742, South Korea

[5] Gyeongsang Natl Univ, Environm Biotechnol Res Ctr, Jinju 660701, South Korea

来源：

FEBS LETTERS | 2005年 / 579卷 / 05期

关键词：

phosphatidylinositol; 3-kinase; phosphatidylinositol(3)phosphate; reactive oxygen species; gravitropism; maize; Arabidopsis thaliana;

D O I：

10.1016/j.febslet.2005.01.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1243 / 1248

页数：6

共 32 条

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