Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

被引：37

作者：

U'Ren, JM

Van Ert, MN

Schupp, JM

Easterday, WR

Simonson, TS

Okinaka, RT

Pearson, T

Keim, P ^{[1
]}

机构：

[1] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA

[2] Los Alamos Natl Lab, Los Alamos, NM 87545 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 11期

关键词：

D O I：

10.1128/JCM.43.11.5771-5774.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

引用

页码：5771 / 5774

页数：4

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