Standardizing immunophenotyping for the Human Immunology Project

被引：820

作者：

Maecker, Holden T. ^{[1
]}

McCoy, J. Philip ^{[2
,3
]}

Nussenblatt, Robert ^{[3
,4
]}

机构：

[1] Stanford Univ, Inst Immun Transplantat & Infect, Sch Med, Stanford, CA 94305 USA

[2] NHLBI, NIH, Bethesda, MD 20892 USA

[3] NIH, Ctr Human Immunol Autoimmun & Inflammat, Bethesda, MD 20892 USA

[4] NEI, NIH, Bethesda, MD 20893 USA

来源：

NATURE REVIEWS IMMUNOLOGY | 2012年 / 12卷 / 03期

关键词：

B-CELL SUBPOPULATIONS; CD8(+) T-CELLS; FLOW-CYTOMETRY; MASS CYTOMETRY; NK CELLS; EXPRESSION; SUBSETS; LYMPHOCYTES; DIFFERENTIATION; ANTIGENS;

D O I：

10.1038/nri3158

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The heterogeneity in the healthy human immune system, and the immunological changes that portend various diseases, have been only partially described. Their comprehensive elucidation has been termed the 'Human Immunology Project'. The accurate measurement of variations in the human immune system requires precise and standardized assays to distinguish true biological changes from technical artefacts. Thus, to be successful, the Human Immunology Project will require standardized assays for immunophenotyping humans in health and disease. A major tool in this effort is flow cytometry, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis. In this Review, we outline the current state of standardization of flow cytometry assays and summarize the steps that are required to enable the Human Immunology Project.

引用

页码：191 / 200

页数：10

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