Aptamer-based colorimetric biosensing of dopamine using unmodified gold nanoparticles

被引：227
作者：
Zheng, Yu [1 ,2 ]
Wang, Yong [1 ,2 ]
Yang, Xiurong [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 156卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Dopamine-binding aptamer; Colorimetry; Gold nanoparticles; Dopamine; Neurotransmitter; CHEMILUMINESCENCE; ACID; NEUROTRANSMITTERS; SEQUENCES; SELECTION; BINDING; GROWTH; PROBES;
D O I：
10.1016/j.snb.2011.03.077
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple, sensitive and selective colorimetric biosensor for the detection of dopamine (DA) was demonstrated with a 58-mer dopamine-binding aptamer (DBA) as recognition element and unmodified gold nanoparticles (AuNPs) as probes. Upon the addition of DA, the conformation of DBA would change from a random coil structure to a rigid tertiary structure like a pocket and this change has been demonstrated by circular dichroism spectroscopic experiments. Besides, the conformational change of DBA could facilitate salt-induced AuNP aggregation and lead to the color change of AuNPs from red to blue. The calibration modeling showed that the analytical linear range covered from 5.4 x 10(-7) M to 5.4 x 10(-6) M and the corresponding limit of detection CLOD) was 3.6x 10(-7) M. Some common interferents such as 3,4-dihydroxyphenylalanine (DOPA), catechol, epinephrine (EP), 3.4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and ascorbic acid (AA) showed no or just a little interference in the determination of DA. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：95 / 99
页数：5
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