Aptamer-based colorimetric biosensing of dopamine using unmodified gold nanoparticles

被引：227
作者：
Zheng, Yu [1 ,2 ]
Wang, Yong [1 ,2 ]
Yang, Xiurong [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 156卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Dopamine-binding aptamer; Colorimetry; Gold nanoparticles; Dopamine; Neurotransmitter; CHEMILUMINESCENCE; ACID; NEUROTRANSMITTERS; SEQUENCES; SELECTION; BINDING; GROWTH; PROBES;
D O I：
10.1016/j.snb.2011.03.077
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple, sensitive and selective colorimetric biosensor for the detection of dopamine (DA) was demonstrated with a 58-mer dopamine-binding aptamer (DBA) as recognition element and unmodified gold nanoparticles (AuNPs) as probes. Upon the addition of DA, the conformation of DBA would change from a random coil structure to a rigid tertiary structure like a pocket and this change has been demonstrated by circular dichroism spectroscopic experiments. Besides, the conformational change of DBA could facilitate salt-induced AuNP aggregation and lead to the color change of AuNPs from red to blue. The calibration modeling showed that the analytical linear range covered from 5.4 x 10(-7) M to 5.4 x 10(-6) M and the corresponding limit of detection CLOD) was 3.6x 10(-7) M. Some common interferents such as 3,4-dihydroxyphenylalanine (DOPA), catechol, epinephrine (EP), 3.4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and ascorbic acid (AA) showed no or just a little interference in the determination of DA. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：95 / 99
页数：5
相关论文
共 33 条
[1]  
[Anonymous], 1996, Circular Dichroism and the Conformational Analysis of Biomolecules
[2]   Dopamine-, L-DOPA-, adrenaline-, and noradrenaline-induced growth of Au nanoparticles: Assays for the detection of neurotransmitters and of tyrosinase activity [J].
Baron, R ;
Zayats, M ;
Willner, I .
ANALYTICAL CHEMISTRY, 2005, 77 (06) :1566-1571
[3]   A simple and rapid HPLC-MS method for the simultaneous determination of epinephrine, norepinephrine, dopamine and 5-hydroxytryptamine: Application to the secretion of bovine chromaffin cell cultures [J].
Carrera, Victoria ;
Sabater, Esther ;
Vilanova, Eugenio ;
Sogorb, Miguel A. .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 847 (02) :88-94
[4]   Enhancement of chemiluminescence of the KlO4-luminol system by gallic acid, acetaldehyde and Mn2+: application for the determination of catecholamines [J].
Chen, Yu-Cheng ;
Lin, Wann-Yin .
LUMINESCENCE, 2010, 25 (01) :43-49
[5]   PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].
GRABAR, KC ;
FREEMAN, RG ;
HOMMER, MB ;
NATAN, MJ .
ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743
[6]   Aptamer-modified gold nanoparticles for colorimetric determination of platelet-derived growth factors and their receptors [J].
Huang, CC ;
Huang, YF ;
Cao, ZH ;
Tan, WH ;
Chang, HT .
ANALYTICAL CHEMISTRY, 2005, 77 (17) :5735-5741
[7]   Electrochemical detection of dopamine in the presence of ascorbic acid using graphene modified electrodes [J].
Kim, Yang-Rae ;
Bong, Sungyool ;
Kang, Yeon-Joo ;
Yang, Yongtak ;
Mahajan, Rakesh Kumar ;
Kim, Jong Seung ;
Kim, Hasuck .
BIOSENSORS & BIOELECTRONICS, 2010, 25 (10) :2366-2369
[8]   Adenosine detection by using gold nanoparticles and designed aptamer sequences [J].
Li, Fan ;
Zhang, Juan ;
Cao, Xuni ;
Wang, Lihua ;
Li, Di ;
Song, Shiping ;
Ye, Bangce ;
Fan, Chunhai .
ANALYST, 2009, 134 (07) :1355-1360
[9]   Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles [J].
Li, HX ;
Rothberg, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (39) :14036-14039
[10]   Label-free colorimetric detection of specific sequences in genomic DNA amplified by the polymerase chain reaction [J].
Li, HX ;
Rothberg, LJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (35) :10958-10961
1234