Synthesis and Identification of Benzoic Acid Artificial Antigen

被引:0
作者
Zhao Wei [1 ]
Wei Chen-xi [1 ]
Xia Ying [1 ]
Tang Bei [1 ]
Yuan Jun-lin [1 ]
机构
[1] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China
来源
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 | 2009年
关键词
benzoic acid; phthalic anhydride; artificial antigen antibody;
D O I
暂无
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The ammonolysis method was used to synthesize the artificial antigen of benzoic acid (BA) by phthalic anhydride (PA), the derivative of benzoic acid (BA), in the present study. BA was linked to ovalbumin (OVA) to generate immunogenic antigen BA-OVA and to bovine serum albumin to generate coating antigen BA-BSA, respectively. Artificial antigen was detected and identified by UV and IR spectral studies, as well as SDS-polyacrylamide gels electrophoresis. BA-OVA was further used to immune BALB/c mice to detect the generation of antibody. Ultraviolet spectrophotometry results showed that the ratio of BA to OVA was 174:1. Antibody was detected in serum of immuned mice by ELISA (The titration of the antiserum is 1: 3.2x10(5)), which suggested that the antigen induced the specific antibody of BA. These results combined suggested that the artificial antigen of BA was synthesized successfully, which makes it possible to establish monoclonal antibodies.
引用
收藏
页码:6046 / 6049
页数:4
相关论文
共 9 条
[1]  
HOLTZAPPLE CK, 1993, J IMMUNOL METHODS, V164, P245
[2]  
LIU FQ, 1998, J AGR BIOTECH, V6, P140
[3]  
LIU ZC, 1989, FOOD NUTR, P200
[4]  
WANG TH, 2005, ANTIBODY THEORY TECH
[5]  
WANG XL, 2002, ORGANIC CHEM
[6]  
*WUX I LIGHT IND, 1985, FOOD AN, P249
[7]  
XING YD, 2005, MOD MED HLTH CHINA, V21, P550
[8]  
YANG LG, 1998, ENZYME IMMUNOASSAY T, P242
[9]  
[杨润亚 Yang Runya], 2004, [西北农林科技大学学报. 自然科学版, Journal of northwest sci-tech university of agriculture and forestry], V32, P64