Novel Cationic Triblock Copolymer of Poly[2-(dimethylamino)ethyl methacrylate]-block-poly(β-amino ester)-block-poly[2-(dimethylamino)ethyl methacrylate]: A Promising Non-Viral Gene Delivery System

被引：17

作者：

Cordeiro, Rosemeyre A. ^{[1
]}

Farinha, Dina ^{[2
]}

Rocha, Nuno ^{[3
]}

Serra, Armenio C. ^{[3
]}

Faneca, Henrique ^{[2
]}

Coelho, Jorge F. J. ^{[2
,3
]}

机构：

[1] Univ Coimbra, Dept Chem Engn, P-3030790 Coimbra, Portugal

[2] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal

[3] Univ Coimbra, Dept Mech Engn, CEMUC, P-3030788 Coimbra, Portugal

来源：

MACROMOLECULAR BIOSCIENCE | 2015年 / 15卷 / 02期

关键词：

cationic block copolymer; click'' chemistry; gene delivery; poly(beta-amino ester); poly[2-(dimethylamino)ethyl methacrylate; MOLECULAR-WEIGHT; POLY(BETA-AMINO ESTER)S; RADICAL POLYMERIZATION; DNA; TRANSFECTION; POLYMERS; SIRNA; NANOPARTICLES; EFFICIENCY; VECTORS;

D O I：

10.1002/mabi.201400424

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This manuscript reports the synthesis of a new cationic block copolymer based on poly[2-(dimethylamino)ethyl methacrylate] and poly(beta-amino ester) from different polymerization strategies. For the first time, it is proposed a triblock copolymer based only on cationic segments, aiming a high biocompatibility, enhanced buffering capacity and stimuli-responsive character in a single structure. The new block copolymer successfully condensed the plasmid DNA into nanosized polyplexes. The polyplexes were tested in two different cell lines revealing similar to 4-fold and similar to 6-fold (in HeLa cells), and similar to 11-fold (in COS-7 cells) higher transgene expression than branched PEI and TurboFect (TM), respectively. These results show that this new block copolymer is a promising candidate to be used as a polymeric non-viral vector.

引用

页码：215 / 228

页数：14

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