Mesencephalic astrocyte-derived neurotrophic factor reduces cell apoptosis via upregulating GRP78 in SH-SY5Y cells

被引：35

作者：

Huang, Jingwei ^{[1
,2
]}

Chen, Changyan ^{[2
]}

Gu, Hua ^{[2
]}

Li, Chen ^{[2
]}

Fu, Xing ^{[3
]}

Jiang, Ming ^{[2
,3
]}

Sun, Hui ^{[1
,2
,3
]}

Xu, Jun ^{[1
]}

Fang, Jianmin ^{[2
,3
]}

Jin, Lingjing ^{[1
]}

机构：

[1] Tongji Univ, Sch Med, Shanghai Tongji Hosp, Dept Neurol, 389 Xincun Rd, Shanghai 200065, Peoples R China

[2] Tongji Univ, Sch Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China

[3] Tongji Univ, Suzhou Inst, Biomed Res Ctr, Bldg 2,198 Jinfeng Rd, Suzhou 215101, Jiangsu, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2016年 / 40卷 / 07期

基金：

中国国家自然科学基金;

关键词：

GRP78; MANF; Parkinson's disease; SH-SY5Y; ENDOPLASMIC-RETICULUM STRESS; UNFOLDED PROTEIN RESPONSE; ALPHA-SYNUCLEIN; NEURON LOSS; RAT MODEL; DEATH; OVEREXPRESSION; PROLIFERATION; MANF; GDNF;

D O I：

10.1002/cbin.10621

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects dopaminergic neurons from damage. In this study, we used MTT, immunohistochemistry, and TUNEL staining to investigate the protective effect of MANF in SH-SY5Y cells treated with 6-OHDA or overexpressed alpha-synuclein. Cleaved caspase-3 levels significantly increased in cells treated with 6-OHDA or overexpressed a-synuclein. 6-OHDA or a alpha-synuclein overexpression that induced cleaved caspase-3 levels to increase was reduced by MANF treatment. In addition, MANF treatment upregulated GRP78 expressions in cells treated with 6-OHDA or overexpressed alpha-synuclein, and RNAi knockdown for GRP78 could block the MANF induced cell survival from 6-OHDA treatment. Furthermore, GRP78 overexpression inhibited 6-OHDA-induced apoptosis. Our data suggest that MANF inhibits apoptosis induced by 6-OHDA and overexpressed a-synuclein in SH-SY5Y cells via upregulating GRP78 in the transcriptional pattern.

引用

页码：803 / 811

页数：9

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