Genome-wide association study and transcriptome analysis reveal new QTL and candidate genes for nitrogen-deficiency tolerance in rice

被引：36

作者：

Li, Qing ^{[1
]}

Lu, Xueli ^{[1
]}

Wang, Changjian ^{[1
]}

Shen, Lan ^{[1
]}

Dai, Liping ^{[1
]}

He, Jinli ^{[1
]}

Yang, Long ^{[1
]}

Li, Peiyuan ^{[1
]}

Hong, Yifeng ^{[1
]}

Zhang, Qiang ^{[1
]}

Dong, Guojun ^{[1
]}

Hu, Jiang ^{[1
]}

Zhang, Guangheng ^{[1
]}

Ren, Deyong ^{[1
]}

Gao, Zhenyu ^{[1
]}

Guo, Longbiao ^{[1
]}

Qian, Qian ^{[1
]}

Zhu, Li ^{[1
]}

Zeng, Dali ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 311401, Zhejiang, Peoples R China

来源：

CROP JOURNAL | 2022年 / 10卷 / 04期

关键词：

Nitrogen; NUE; Rice; GWAS; RNA-seq; USE EFFICIENCY; AGRONOMIC TRAITS; USE DIVERGENCE; NITRATE; AGRICULTURE; PROGRAM; INDICA; PLANTS; MODEL; STAGE;

D O I：

10.1016/j.cj.2021.12.006

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The development of rice cultivars with improved nitrogen use efficiency (NUE) is desirable for sustain-able agriculture. Achieving this goal depends in part on understanding how rice responds to low soil nitrogen (N) and identifying causative genes underlying this trait. To identify quantitative trait loci (QTL) or genes associated with low N response, we conducted a genome-wide association study (GWAS) using a diverse panel of 230 rice accessions and performed a transcriptomic investigation of rice accessions with differential responses to low N stress at two N levels. We detected 411 GWAS-associated genes in 5 QTL and 2722 differentially expressed genes in response to low N, of which 24 were identified by both methods and ranked according to gene annotations, literature queries, gene expression, and genetic diversity analysis. The large-scale datasets obtained from this study reveal low N-responsive characteristics and provide insights towards understanding the regulatory mechanisms of N-deficiency tolerance in rice, and the candidate genes or QTL would be valuable resources for increasing rice NUE via molecular biotechnology. (c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：942 / 951

页数：10

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