Age-and gender-related pattern of methylation in the MT-RNR1 gene

被引：29

作者：

D'Aquila, Patrizia ^{[1
]}

Giordano, Marco ^{[1
]}

Montesanto, Alberto ^{[1
]}

De Rango, Francesco ^{[1
]}

Passarino, Giuseppe ^{[1
]}

Bellizzi, Dina ^{[1
]}

机构：

[1] Univ Calabria, Dept Biol Ecol & Earth Sci DIBEST, I-87036 Arcavacata Di Rende, Italy

来源：

EPIGENOMICS | 2015年 / 7卷 / 05期

关键词：

12S ribosomal RNA gene; 12S ribosomal RNA gene methylation; 16S ribosomal RNA gene; aging; DNA methylation; mitochondrial DNA; MT-RNR1; gene; mortality risk; MITOCHONDRIAL-DNA METHYLATION; MAMMALIAN MITOCHONDRIA; EPIGENETIC REGULATION; VARIANTS; SEQUENCE; SHOWS; MTDNA;

D O I：

10.2217/epi.15.30

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We evaluated the methylation of two CpG sites located within human mitochondrial 12S and 16S ribosomal RNA (MT-RNR1 and MT-RNR2) genes. Materials & methods: Methylation was measured through bisulfite sequencing and qPCR assays on DNA samples collected from 381 differently aged human subjects. Results: Analyses revealed the methylation of the site in the MT-RNR1 gene and the co-presence of both unmethylated and methylated cytosines in most samples. High methylation levels (>10%) were more frequent in old women with respect to younger controls. A 9-year-long follow-up survey showed that subjects with high methylation levels exhibit a mortality risk significantly higher than subjects with low levels. Conclusion: Our data further support the presence of methylation within human mitochondrial DNA and suggest that high levels of methylation of the MT-RNR1 site may reflect a condition of the cell or of the organism unfavorable to survival.

引用

页码：707 / 716

页数：10

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