Improvement of strain discrimination by combination of RAPD with PFGE for the analysis of the swine isolates of Salmonella enterica serovar Choleraesuis

被引：10

作者：

Chiu, Tsai-Hsin ^{[2
]}

Pang, Jen-Chieh ^{[3
]}

Chen, Ming-Hui ^{[1
]}

Tsen, Hau-Yang ^{[1
]}

机构：

[1] Hung Kuang Univ, Dept Food Sci & Appl Biotechnol, Taichung Cty, Taiwan

[2] Natl Peng Hu Univ, Dept Food Sci, Peng Hu, Taiwan

[3] Van Nung Univ, Dept Biotechnol, Tao Yuan, Taiwan

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2011年 / 27卷 / 02期

关键词：

Salmonella enterica serovar Choleraesuis; Swine isolates; PFGE; RAPD; FIELD GEL-ELECTROPHORESIS; PIGS; DIFFERENTIATION; TAIWAN;

D O I：

10.1007/s11274-010-0467-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Salmonella enterica serovar Choleraesuis (S. Choleraesuis) can cause salmonellosis in pigs and humans. Currently, the most common method used for the subtyping of this Salmonella serovar is pulsed field gel electrophoresis (PFGE) using XbaI as a DNA digestion enzyme. In this study, we compared and combined PFGE with the randomly amplified polymorphic DNA method, for the typing of 95 S. Choloraesuis strains isolated from diseased pigs. Using PFGE with XbaI, with AvrII, and with SpeI digested DNA, 29, 74, and 40 patterns, respectively, were obtained. Also, 53, 15, and 35 strains, respectively, belong to the major patterns X1, A1, and S1. When these three digestion patterns were combined, 83 PFGE pattern combinations were obtained. On the other hand, using RAPD with selected primer alone generated 76 patterns, and 11 strains which fell within a single X1A1S1 PFGE combination pattern were discriminated into 10 patterns. Thus, for S. Choloraesuis, PFGE with AvrII allowed higher discrimination than PFGE with XbaI, and some of the PFGE groupings obtained by combining the XbaI, AvrII and SpeI digestion patterns were further subdivided by the RAPD method.

引用

页码：465 / 469

页数：5

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