The nuclear genes encoding the internal (KlNDI1) and external (KlNDE1) alternative NAD(P)H:ubiquinone oxidoreductases of mitochondria from Kluyveromyces lactis

被引：29

作者：

Tarrío, N ^{[1
]}

Prado, SD ^{[1
]}

Cerdán, ME ^{[1
]}

Siso, MIG ^{[1
]}

机构：

[1] Univ A Coruna, Fac Ciencias, Dept Mol & Cell Biol, La Coruna 15071, Spain

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2005年 / 1707卷 / 2-3期

关键词：

NAD(P)H : ubiquinone oxidoreductase; alternative dehydrogenase; Kluyveromyces lactis; NDI1; NDE1; mitochondrial respriation;

D O I：

10.1016/j.bbabio.2004.12.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cloning, sequence and functional analyses of the Kluyveromyces lactis genes KlNDI1 and KlNDE1 are reported. These genes encode for proteins with high homology to the mitochondrial internal (Ndi1p) and external (Nde1p) alternative NADH:ubiquinone oxidoreductases from Saccharomyces cerevisiae and complement the respective mutations. Analysis of KlNDI1 transcriptional regulation showed that expression of this gene is lower in 2% glucose than in 0.5% glucose or non-fermentable carbon sources. Beta-galactosidase activity values, shown by lacZ fusions of KlNDI1 promoter deletions, suggested that two Adr1p binding sites mediate this carbon source regulation of KlNDI1. The expression of the KlNDE1 gene in S. cerevisiae mutant strains and measurement of respiration with isolated mitochondria showed that the protein encoded by KlNDE1 oxidizes NADPH, this being an important difference with respect to the conventional yeast S. cerevisiae. Moreover, Northern blot experiments using a phosphoglucose isomerase mutant showed that KlNDE1 gene transcription increases with glucose metabolism through the pentose phosphate pathway. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：199 / 210

页数：12