Determination of catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography and electrochemical detection

被引:32
作者
Jiang, Liwei [1 ]
Chen, Yibang [1 ]
Luo, Yanmei [1 ]
Tan, Yueming [1 ]
Ma, Ming [1 ]
Chen, Bo [1 ]
Xie, Qingji [1 ]
Luo, Xubiao [2 ]
机构
[1] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ China, Key Lab Phytochem R&D Hunan Prov, Changsha 410081, Hunan, Peoples R China
[2] Nanchang Hangkong Univ, Key Lab Jiangxi Prov Persistent Pollutants Contro, Nanchang, Peoples R China
基金
中国国家自然科学基金;
关键词
Catecholamines; Boronate affinity interaction; Electrochemical detection; Magnetic nanoparticles; Sample preparation; SOLID-PHASE EXTRACTION; TANDEM MASS-SPECTROMETRY; SELECTIVE ENRICHMENT; FACILE SYNTHESIS; BIOGENIC-AMINES; DOPAMINE; MICROEXTRACTION; METABOLITES; SEPARATION; SAMPLES;
D O I
10.1002/jssc.201400920
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new method was developed for the simultaneous determination of three catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography with electrochemical detection. Novel aminophenylboronic acid functionalized magnetic nanoparticles were prepared by multi-step covalent modification, and characterized by transmission electron microscopy, Fourier-transformed infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometry. With the help of the high affinity between the boronate and cis-diol group, the particles were used for the highly selective separation and enrichment of three major catecholamines, norepinephrine, epinephrine, and dopamine. Effects of the pH of the feed solution, the extraction time, the composition of the buffer solution, the amount of the magnetic particles, the elution conditions, and the recycling of aminophenylboronic acid functionalized magnetic nanoparticles were explored. Under the optimized conditions, 13-17-fold enrichment factors were obtained. The linear ranges were 0.01-2.0 mu g/mL for the studied analytes. The limits of detection and quantification were in the range of 2.0-7.9 and 6.7-26.3 ng/mL, respectively. The relative recoveries were in the range of 92-108%, with intraday and inter-day relative standard deviations lower than 6.8%. This method was successfully applied to analysis of catecholamines in real urine.
引用
收藏
页码:460 / 467
页数:8
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