LncRNA PICSAR binds to miR-485-5p and activates TGF-β1/Smad to promote abnormal proliferation of hypertrophic scar fibroblasts (HSFs) and excessive deposition of extracellular matrix (ECM)

被引：10

作者：

Xu, Sijia ^{[1
]}

Dong, Wenxin ^{[1
]}

Shi, Yu ^{[1
]}

机构：

[1] Shanghai Skin Dis Hosp, Dept Med Cosmet Ctr, 1278 Baode Rd, Shanghai 200443, Peoples R China

来源：

MEDICAL MOLECULAR MORPHOLOGY | 2021年 / 54卷 / 04期

关键词：

LncRNA PICSAR; miR-485-5p; Hypertrophic scar fibroblasts; ECM; TGF-beta; 1; Smad; LONG NONCODING RNA; TGF-BETA/SMAD; INVASION;

D O I：

10.1007/s00795-021-00296-4

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The purpose of this study is to explore whether LncRNA PICSAR binds to miR-485-5p and thereby activates TGF-beta 1/Smad signaling pathway, influencing the abnormal proliferation of fibroblasts and excessive deposition of ECM in hypertrophic scar formation. PICSAR and miR-485-5p expressions were detected by qPCR. Cell proliferation was examined by CCK-8. Protein expressions were determined by western blot. Immunofluorescence detected the Ki-67 expression. Dual-luciferase followed by immunoprecipitation was performed to verify the interaction between PICSAR and miR-485-5p. Interference with PICSAR inhibited the abnormal proliferation of hypertrophic scar fibroblasts (HSFs) and the excessive deposition of ECM. It was also confirmed in our study that MiR-485-5p is a direct target of PICSAR in HSFs. Additionally, inhibition of miR-485-5p reversed the effect of PICSAR knockdown in HSFs. LncRNA PICSAR binds to miR-485-5p and thereby activates TGF-beta 1/Smad signaling pathway, promoting the abnormal proliferation of fibroblasts and excessive deposition of ECM in hypertrophic scar formation.

引用

页码：337 / 345

页数：9

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