Comparison of ELISA with other serological tests in the diagnosis of ovine brucellosis

被引:0
|
作者
Öngör, H [1 ]
Muz, A [1 ]
Çetinkaya, B [1 ]
机构
[1] Firat Univ, Vet Fak, Mikrobiyol Anabilim Dali, TR-23119 Elazig, Turkey
来源
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | 2001年 / 25卷 / 01期
关键词
brucellosis; ELISA; serological tests; sheep; Brucella melitensis;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In this study, antibodies against Brucella melitensis in aborted sheep were investigated with enzyme-linked immunosorbent assay (ELISA), standard tube agglutination test (SAT), rose bengal plate test (RBPT), complement fixation test (CFT), 2-mercaptoethanol microagglutination test (2-ME-MAT) and dithiothreitol microagglutination test (DTT-MAT). The sensitivity and specificity of the tests were calculated by using CFT results as the gold standard. A Kappa value was used to measure the agreements between ELISA and the other tests. Sonicated antigen prepared from Brucella melitensis 16M strain, the peroxidase labelled anti-sheep IgG conjugate and orthophenylenediamine substrate were used in ELISA. A total of 500 blood samples collected from 36 sheep flocks in Elazig and environs were examined, One hundred and three (20.6%) samples in ELISA. 55 (11%) in RBPT, 84 (16.8%) in SAT, 89 (17.8%) in CFT, 79 (15.8%) in 2-ME-MAT and 81 (16.2%) in DTT MAT were found to be brucella-positive. In the comparison of these tests, a significant difference was detected between ELISA and RBPT results (p<0.001), whereas the differences between ELISA and other tests were not statistically significant (p>0.05), Excel lent agreement was detected between ELISA and CFT, DTT-MAT, 2-ME-MAT and SAT results. with kappa values of 88%, 84%. 83% and 85%, respectively. However, in the comparison of ELISA with RBPT, a kappa value of 64% (fair agreement) was obtained. in addition. the sensitivity and specificity of ELISA were determined to be 97% and 96%, respectively.
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页码:21 / 26
页数:6
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