Mouse B-Type Lamins Are Required for Proper Organogenesis But Not by Embryonic Stem Cells

被引：192
作者：
Kim, Youngjo [2 ,3 ]
Sharov, Alexei A. [1 ]
McDole, Katie [3 ,4 ]
Cheng, Melody [5 ]
Hao, Haiping [6 ]
Fan, Chen-Ming [2 ,4 ]
Gaiano, Nicholas [5 ]
Ko, Minoru S. H. [1 ]
Zheng, Yixian [2 ,3 ,4 ]
机构：
[1] NIA, Dev Genom & Aging Sect, Genet Lab, NIH, Baltimore, MD 21224 USA
[2] Carnegie Inst Sci, Dept Embryol, Baltimore, MD 21218 USA
[3] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA
[4] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
[5] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
[6] Johns Hopkins Univ, Sch Med, Microarray Core Facil, Baltimore, MD 21205 USA
来源：
SCIENCE | 2011年 / 334卷 / 6063期
关键词：
NUCLEAR LAMINA; DIFFERENTIATION; ORGANIZATION; EXPRESSION; GENE; PROLIFERATION; PERIPHERY; DEFECTS;
D O I：
10.1126/science.1211222
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not directly required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons probably cause brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish a foundation for redefining the function of the nuclear lamina in the context of tissue building and homeostasis.
引用
收藏
页码：1706 / 1710
页数：5
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