Characterization of orthopoxviruses isolated from man and animals in Germany

被引:36
作者
Meyer, H
Schay, C
Mahnel, H
Pfeffer, M
机构
[1] Fed Armed Forces Med Acad, Inst Microbiol, Munich, Germany
[2] Univ Munich, Inst Med Microbiol Infect & Epidem Dis, Munich, Germany
关键词
D O I
10.1007/s007050050520
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Fourteen orthopoxvirus strains isolated from humans, cats, a dog, a cow, and an elephant in Germany were characterized. All were classified as cowpox virus based on haemorrhagic lesions induced on the chorioallantoic membrane of chicken eggs and reactivity of a 160 kDa protein with anti-A-type inclusion protein hyperimmun serum in a Western blot. More detailed comparison of the isolates by restriction endonuclease mapping using HindIII and XhoI demonstrated a close relationship between all isolates and confirmed them as cowpox viruses. However, some minor differences between the isolates were detected which proved to be of epidemiological value. One group consisting of five closely related isolates contained a unique 4.0 kb HindIII fragment. In a Southern blot this fragment failed to hybridize with other cowpox virus isolates including the reference strain.
引用
收藏
页码:491 / 501
页数:11
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