Glucagon-like peptide 1 (GLP-1) has great potential in diabetes therapy. In order to accumulate GLP-1 in endosperm tissue of rice, a codon-optimized GLP-1 (mGLP-1) synthetic gene was directly expressed under the control of rice storage protein glutelin GluB-1 promoter in transgenic rice plants. Unexpectedly, neither the transcripts nor the transgene products were detected in the seeds of regenerated plants. Furthermore, transcripts of GluB-1 gene in these transgenic plants were not detected, and small interfering RNAs (siRNAs) corresponding to the transgene were detected. These results indicated that the expression of mGLP-1 was silenced by co-suppression in rice transgenic seeds. To avoid silencing, mGLP-1 was fused to GFP with or without self-processing 2A sequence, and introduced into rice plants. Both chimeric genes were highly expressed in these transgenic rice seeds, indicating that gene silencing could be avoided by changing the transgene components. Furthermore, the fusion protein containing the 2A sequence were processed into GFP-2A and mGLP-1 peptides with the efficiency of more than 80%, but the processed mGLP-1 peptides were not detected. Lack of accumulation of mGLP-1 may be explained by proteolytic digestion in the cytoplasm.
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Univ Szeged, Dept Med Chem, Szeged, HungaryUniv Szeged, Dept Med Chem, Szeged, Hungary
Szolomajer, Janos
Kele, Zoltan
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Univ Szeged, Dept Med Chem, Szeged, HungaryUniv Szeged, Dept Med Chem, Szeged, Hungary
Kele, Zoltan
Toth, Gabor K.
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Univ Szeged, Dept Med Chem, Szeged, HungaryUniv Szeged, Dept Med Chem, Szeged, Hungary
Toth, Gabor K.
Straner, Pal
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Eotvos Lorand Univ, MTA ELTE Prot Model Res Grp, Budapest, Hungary
Lab Struct Chem & Biol, Budapest, HungaryUniv Szeged, Dept Med Chem, Szeged, Hungary
Straner, Pal
Perczel, Andras
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Eotvos Lorand Univ, MTA ELTE Prot Model Res Grp, Budapest, Hungary
Lab Struct Chem & Biol, Budapest, HungaryUniv Szeged, Dept Med Chem, Szeged, Hungary
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Univ Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USAUniv Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USA
Gier, B.
Butler, P. C.
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Univ Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USAUniv Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USA
Butler, P. C.
Lai, C. K.
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Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USAUniv Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USA
Lai, C. K.
DeNicola, M. M.
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Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USAUniv Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USA
DeNicola, M. M.
Yeh, M. W.
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Univ Calif Los Angeles, David Geffen Sch Med, Dept Surg, Endocrine Surg Unit, Los Angeles, CA 90095 USAUniv Calif Los Angeles, David Geffen Sch Med, Larry Hillblom Islet Res Ctr, Los Angeles, CA 90095 USA