Starting a new life: Sperm PLC-zeta mobilizes the Ca2+signal that induces egg activation and embryo development

被引：63

作者：

Nomikos, Michail ^{[1
,2
]}

Swann, Karl ^{[1
]}

Lai, F. Anthony ^{[1
]}

机构：

[1] Cardiff Univ, Cell Signalling Lab, WHRI, Inst Mol & Expt Med,Sch Med, Cardiff, S Glam, Wales

[2] Natl Ctr Sci Res Demokritos, IRRP, Athens, Greece

来源：

BIOESSAYS | 2012年 / 34卷 / 02期

基金：

英国惠康基金;

关键词：

calcium signalling; fertilization; oocyte activation; phospholipase C; sperm; PHOSPHOLIPASE-C-ZETA; INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; PLECKSTRIN-HOMOLOGY-DOMAIN; TRIGGERS CA2+ OSCILLATIONS; MOUSE EGGS; CALCIUM OSCILLATIONS; PARTHENOGENETIC ACTIVATION; HAMSTER EGGS; MAMMALIAN FERTILIZATION; NUCLEAR TRANSLOCATION;

D O I：

10.1002/bies.201100127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have discovered that a single sperm protein, phospholipase C-zeta (PLC?), can stimulate intracellular Ca2+ signalling in the unfertilized oocyte (egg) culminating in the initiation of embryonic development. Upon fertilization by a spermatozoon, the earliest observed signalling event in the dormant egg is a large, transient increase in free Ca2+ concentration. The fertilized egg responds to the intracellular Ca2+ rise by completing meiosis. In mammalian eggs, the Ca2+ signal is delivered as a train of long-lasting cytoplasmic Ca2+ oscillations that begin soon after gamete fusion and persist beyond the completion of meiosis. Sperm PLC? effects Ca2+ release from egg intracellular stores by hydrolyzing the membrane lipid PIP2 and consequent stimulation of the inositol 1,4,5-trisphosphate (InsP3) receptor Ca2+-signalling pathway, leading to egg activation and early embryogenesis. Recent advances have refined our understanding of how PLC? induces Ca2+ oscillations in the egg and also suggest its potential dysfunction as a cause of male infertility.

引用

页码：126 / 134

页数：9

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