Inhibitory activity on matrix metalloproteinases and cell-proliferating activity of tissue inhibitor of metalloproteinases-1 (TIMP-1) - contrastive difference between human and bovine TIMP-1s on mouse cell proliferation

被引：5

作者：

Nishimura, K

Yamashita, K

Kato, Y

Iwata, T

Iwata, K

Nishikawa, T

Goto, S

Hayakawa, T

机构：

[1] Aichi Gakuin Univ, Sch Dent, Dept Biochem, Chikusa Ku, Nagoya, Aichi 4648650, Japan

[2] Aichi Gakuin Univ, Sch Dent, Dept Orthodont, Chikusa Ku, Nagoya, Aichi 4648650, Japan

[3] Daiichi Fine Chem Co Ltd, Strateg Dev Dept, Toyama, Japan

来源：

GROWTH FACTORS | 2005年 / 23卷 / 02期

关键词：

human and bovine TIMP-1; MMP inhibitory activity; cell-growth promoting activity; tyrosine phosphorylation; ERK; human and mouse cells;

D O I：

10.1080/08977190500138772

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Either human or bovine TIMP-1 inhibited matrix metalloproteinase (MMP) activities, such as collagenolytic, gelatinolytic and caseinolytic, expressed by mouse cells as well as those expressed by human cells. Bovine TIMP-1 stimulated the proliferation of both human and mouse cells, but human TIMP-1 only proliferate human cells and not mouse cells indicating that the cell-proliferating activity has more strict species specificity than MMP inhibitory activity. All the results from [H-3]thymidine incorporation, the phosphorylation of tyro sine-containing cellular proteins and extracellular-signal-regulated protein kinases supported the cell-proliferating properties of both human and bovine TIMP-1s. Human TIMP-1 seems not to bind to TIMP-1 receptors on mouse cells.

引用

页码：135 / 142

页数：8