An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue

被引:116
|
作者
Goodpaster, Tracy [2 ]
Legesse-Miller, Aster [1 ]
Harneed, Meera R. [3 ]
Aisner, Seena C. [3 ]
Randolph-Habecker, Julie [2 ]
Coller, Hilary A. [1 ]
机构
[1] Princeton Univ, Lewis Thomas Lab, Dept Mol Biol, Princeton, NJ 08544 USA
[2] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA
[3] Univ Med & Dent New Jersey, Dept Pathol, Newark, NJ 07103 USA
关键词
fibroblasts; immunohistochemistry; TE-7;
D O I
10.1369/jhc.7A7287.2007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors.
引用
收藏
页码:347 / 358
页数:12
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