Localized CT-Guided Irradiation Inhibits Neurogenesis in Specific Regions of the Adult Mouse Brain

被引：48

作者：

Ford, E. C. ^{[1
]}

Achanta, P. ^{[3
]}

Purger, D. ^{[3
]}

Armour, M. ^{[1
]}

Reyes, J. ^{[1
]}

Fong, J. ^{[1
]}

Kleinberg, L. ^{[1
]}

Redmond, K. ^{[1
]}

Wong, J. ^{[1
]}

Jang, M. H. ^{[4
,5
]}

Jun, H. ^{[4
]}

Song, H-J. ^{[4
,5
,6
]}

Quinones-Hinojosa, A. ^{[2
,3
]}

机构：

[1] Johns Hopkins Univ, Dept Radiat Oncol & Mol Radiat Sci, Sch Med, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Dept Oncol, Sch Med, Baltimore, MD 21231 USA

[3] Johns Hopkins Univ, Dept Neurosurg, Sch Med, Baltimore, MD 21231 USA

[4] Johns Hopkins Univ, Inst Cell Engn, Sch Med, Baltimore, MD 21231 USA

[5] Johns Hopkins Univ, Dept Neurol, Sch Med, Baltimore, MD 21231 USA

[6] Johns Hopkins Univ, Dept Neurosci, Sch Med, Baltimore, MD 21231 USA

来源：

RADIATION RESEARCH | 2011年 / 175卷 / 06期

基金：

美国国家卫生研究院;

关键词：

RADIATION RESEARCH PLATFORM; ANIMAL CONFORMAL IRRADIATOR; HIPPOCAMPAL NEUROGENESIS; DENTATE GYRUS; SUBVENTRICULAR ZONE; X-IRRADIATION; CRANIAL IRRADIATION; H2AX PHOSPHORYLATION; IONIZING-RADIATION; MAMMALIAN BRAIN;

D O I：

10.1667/RR2214.1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Radiation is used in the study of neurogenesis in the adult mouse both as a model for patients undergoing radiation therapy for CNS malignancies and as a tool to interrupt neurogenesis. We describe, the use of a dedicated CT-guided precision device to irradiate specific sub-regions of the adult mouse brain. Improved CT visualization was accomplished with intrathecal injection of iodinated contrast agent, which enhances the lateral ventricles. T2-weighted MRI images were also used for target localization. Visualization of delivered beams (10 Gy) in tissue was accomplished with immunohistochemical staining for the protein gamma-H2AX, a marker of DNA double-strand breaks. gamma-H2AX stains showed that the lateral ventricle wall could be targeted with an accuracy of 0.19 mm (n = 10). In the hippocampus, gamma-H2AX staining showed that the dentate gyrus can be irradiated unilaterally with a localized arc treatment. This resulted in a significant decrease of proliferative neural progenitor cells as measured by Ki-67 staining (P < 0.001) while leaving the contralateral side intact. Two months after localized irradiation, neurogenesis was significantly inhibited in the irradiated region as seen with EdU/NeuN double labeling (P < 0.001). Localized radiation in the rodent brain is a promising new tool for the study of neurogenesis. (C) 2011 by Radiation Research Society

引用

页码：774 / 783

页数：10

共 61 条

[61] High-resolution, small animal radiation research platform with X-ray tomographic guidance capabilities
Wong, John
Armour, Elwood
Kazanzides, Peter
Iordachita, Ulian
Tryggestad, Erik
Deng, Hua
Matinfar, Mohammad
Kennedy, Christopher
Liu, Zejian
Chan, Timothy
Gray, Owen
Verhaegen, Frank
McNutt, Todd
Ford, Eric
DeWeese, Theodore L.
[J]. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2008, 71 (05): : 1591 - 1599

← 1 2 3 4 5 6 7 →