Effects of Virus-associated Molecular Patterns on the Expression of Cathelicidins in the Hen Vagina

被引：8

作者：

Abdel-Mageed, Ahmad M. ^{[1
,2
]}

Isobe, Naoki ^{[2
,3
]}

Yoshimura, Yukinori ^{[2
,3
]}

机构：

[1] Menia Univ, Fac Sci, Al Minya 61519, Egypt

[2] Hiroshima Univ, Grad Sch Biosphere Sci, Higashihiroshima 7398528, Japan

[3] Hiroshima Univ, Res Ctr Anim Sci, Higashihiroshima 7398528, Japan

来源：

JOURNAL OF POULTRY SCIENCE | 2016年 / 53卷 / 03期

基金：

日本学术振兴会;

关键词：

cathelicidins; hen oviduct; Toll-like receptor ligands; virus-associated molecular patterns; AVIAN BETA-DEFENSINS; TOLL-LIKE RECEPTORS; FUNCTIONAL-CHARACTERIZATION; PROINFLAMMATORY CYTOKINES; CPG OLIGODEOXYNUCLEOTIDES; ANTIMICROBIAL PEPTIDES; EGGSHELL FORMATION; MAMMALIAN TLR9; CHICKEN TLR21; MAMMARY-GLAND;

D O I：

10.2141/jpsa.0150180

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of this study was to examine the expression profiles of the cathelicidins (CATHs) in the oviduct and the effects of Toll-like receptor (TLR) ligands of virus-associated molecular patterns on CATHs expression in the vagina of hens. The mRNA expression of cathelicidins (CATH1, -2, -3 and -B1) in the oviductal mucosa was analyzed by RT-PCR. The effects of viral moleculs on the CATHs expression in the vagina was examined by incubating the mucosal tissue with virus molecular patterns, including poly I:C (dsRNA virus, TLR3 ligand), R848 (ssRNA virus, TLR7 ligand) and CpG-ODN (DNA virus, TLR21 ligand), followed by real-time PCR analysis. The expression of CATH1, CATH2 and CATH3 was identified in all oviductal segments, except for CATH2 which was lacked in the magnum. The expression of CATHB1 was not identified at any segments of the oviduct. Poly I:C down-regulated the expression of CATH1, -2 and -3, whereas R848 up-regulated the expression of CATH1 and CATH3 but down-regulated the expression of CATH2. CpG-ODN did not affect the CATHs expression. These results suggest that mucosal tissues of the oviduct express CATHs to provide the defense mechanism against microbes, and the expression of CATH1 and CATH3 is up-regulated against ssRNA viruses, whereas, dsRNA virus may suppress the expression of CATH1, -2 and -3.

引用

页码：240 / 247

页数：8

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