Replication and single-cycle delivery of SARS-CoV-2 replicons

被引：52

作者：

Ricardo-Lax, Inna ^{[1
]}

Luna, Joseph M. ^{[1
]}

Thao, Tran Thi Nhu ^{[2
,3
,4
]}

Le Pen, Jeremie ^{[1
]}

Yu, Yingpu ^{[1
]}

Hoffmann, H-Heinrich ^{[1
]}

Schneider, William M. ^{[1
]}

Razooky, Brandon S. ^{[1
]}

Fernandez-Martinez, Javier ^{[5
]}

Schmidt, Fabian ^{[6
]}

Weisblum, Yiska ^{[6
]}

Trueb, Bettina Salome ^{[2
,3
]}

Veiga, Ines Berenguer ^{[2
,3
]}

Schmied, Kimberly ^{[2
,3
]}

Ebert, Nadine ^{[2
,3
]}

Michailidis, Eleftherios ^{[1
]}

Peace, Avery ^{[1
]}

Sanchez-Rivera, Francisco J. ^{[7
]}

Lowe, Scott W. ^{[7
]}

Rout, Michael P. ^{[5
]}

Hatziioannou, Theodora ^{[6
]}

Bieniasz, Paul D. ^{[6
]}

Poirier, John T. ^{[8
]}

MacDonald, Margaret R. ^{[1
]}

Thiel, Volker ^{[2
,3
]}

Rice, Charles M. ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Virol & Infect Dis, New York, NY 10065 USA

[2] Inst Virol & Immunol IVI, Bern, Switzerland

[3] Univ Bern, Vet Fac, Dept Infect Dis & Pathobiol, Bern, Switzerland

[4] Univ Bern, Grad Sch Biomed Sci, Bern, Switzerland

[5] Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA

[6] Rockefeller Univ, Lab Retrovirol, New York, NY 10065 USA

[7] MSKCC, Canc Biol & Genet, New York, NY 10065 USA

[8] NYU, Grossman Sch Med, NYU Langone Hlth, Laura & Isaac Perlmutter Canc Ctr, New York, NY 10016 USA

来源：

SCIENCE | 2021年 / 374卷 / 6571期

基金：

美国国家卫生研究院; 瑞士国家科学基金会; 美国国家科学基金会;

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; PHI-29; DNA-POLYMERASE; HEPATITIS-C VIRUS; GENE-EXPRESSION; RNA REPLICATION; AMPLIFICATION; NSP1; IDENTIFICATION; ESTABLISHMENT; CONSTRUCTION;

D O I：

10.1126/science.abj8430

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be transcomplemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.

引用

页码：1099 / +

页数：29

共 50 条

[1] Cell Entry and Unusual Replication of SARS-CoV-2
McCann, Nathan
Castellino, Francis J.
CURRENT DRUG TARGETS, 2022, 23 (17) : 1539 - 1554
[2] SARS-CoV-2 replication and drug discovery
Nazir, Farah
Kombe, Arnaud John Kombe
Khalid, Zunera
Bibi, Shaheen
Zhang, Hongliang
Wu, Songquan
Jin, Tengchuan
MOLECULAR AND CELLULAR PROBES, 2024, 77
[3] Viroporins: Structure, function, and their role in the life cycle of SARS-CoV-2
Breitinger, Ulrike
Farag, Noha S.
Sticht, Heinrich
Breitinger, Hans-Georg
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2022, 145
[4] Targeting ACLY efficiently inhibits SARS-CoV-2 replication
Yuen, Terrence Tsz-Tai
Chan, Jasper Fuk-Woo
Yan, Bingpeng
Shum, Cynthia Cheuk-Ying
Liu, Yuanchen
Shuai, Huiping
Hou, Yuxin
Huang, Xiner
Hu, Bingjie
Chai, Yue
Yoon, Chaemin
Zhu, Tianrenzheng
Liu, Huan
Shi, Jialu
Zhang, Jinjin
Cai, Jian-Piao
Zhang, Anna Jinxia
Zhou, Jie
Yin, Feifei
Yuan, Shuofeng
Zhang, Bao-Zhong
Chu, Hin
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2022, 18 (12): : 4714 - 4730
[5] Fidelity of Ribonucleotide Incorporation by the SARS-CoV-2 Replication Complex
Yin, Xingyu
Popa, Horia
Stapon, Anthony
Bouda, Emilie
Garcia-Diaz, Miguel
JOURNAL OF MOLECULAR BIOLOGY, 2023, 435 (05)
[6] The SARS-CoV-2 RNA interactome
Lee, Sungyul
Lee, Young-suk
Choi, Yeon
Son, Ahyeon
Park, Youngran
Lee, Kyung-Min
Kim, Jeesoo
Kim, Jong-Seo
Kim, V. Narry
MOLECULAR CELL, 2021, 81 (13) : 2838 - +
[7] In the eye of the storm: SARS-CoV-2 infection and replication at the ocular surface?
Armstrong, Lyle
Collin, Joseph
Mostafa, Islam
Queen, Rachel
Figueiredo, Francisco C.
Lako, Majlinda
STEM CELLS TRANSLATIONAL MEDICINE, 2021, 10 (07) : 976 - 986
[8] New Chemicals Suppressing SARS-CoV-2 Replication in Cell Culture
Sulimov, Alexey
Ilin, Ivan
Kutov, Danil
Shikhaliev, Khidmet
Shcherbakov, Dmitriy
Pyankov, Oleg
Stolpovskaya, Nadezhda
Medvedeva, Svetlana
Sulimov, Vladimir
MOLECULES, 2022, 27 (17):
[9] Characterization of SARS-CoV-2 replication complex elongation and proofreading activity
Jones, Alisha N.
Mourao, Andre
Czarna, Anna
Matsuda, Alex
Fino, Roberto
Pyrc, Krzysztof
Sattler, Michael
Popowicz, Grzegorz M.
SCIENTIFIC REPORTS, 2022, 12 (01)
[10] Inhibitors of SARS-CoV-2 PLpro
Calleja, Dale J.
Lessene, Guillaume
Komander, David
FRONTIERS IN CHEMISTRY, 2022, 10

← 1 2 3 4 5 →