Effective Agrobacterium-mediated transformation of chicory (Cichorium intybus L.) with Mycobacterium tuberculosis antigene ESAT6

被引：3
|
作者：
Matvieieva, N. A. [1 ]
Vasylenko, M. Y. [1 ]
Shahovsky, A. M. [1 ]
Bannykova, M. O. [1 ]
Kvasko, O. Y. [1 ]
Kuchuk, N. V. [1 ]
机构：
[1] Natl Acad Sci Ukraine, Inst Cell Biol & Genet Engn, Kiev, Ukraine
来源：
CYTOLOGY AND GENETICS | 2011年 / 45卷 / 01期
关键词：
TOXIN-B-SUBUNIT; TRANSGENIC PLANTS; EDIBLE VACCINE; EXPRESSION; IMMUNOGENICITY; REGENERATION; EFFICACY; ANTIBODIES; OLIGOMERS; PROGRESS;
D O I：
10.3103/S0095452711010038
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Conditions of high efficient chicory (Cichorium intybus L.) transformation with Mycobacterium tuberculosis antigene ESAT6 responsible for the maximal frequency of regeneration of green plants with transformed DNA have been determined. Transformation frequency was up to 86% when the cotyledons were cultivated within 3 days without cefotaxime followed by 1 day of cultivation without kanamycine. According to PCR-analysis, the presence of both selective nptII and target esxA genes was shown in all analyzed plants. At the same time, RT-PCR analysis demonstrated the presence of nptII transcripts in eight analyzed lines and esxA transcripts in only five analyzed lines.
引用
收藏
页码：7 / 12
页数：6
相关论文
共 50 条
  • [1] AGROBACTERIUM TUMEFACIENS MEDIATED TRANSFORMATION OF CHICORY (CICHORIUM INTYBUS L.) WITH MYTILUS GALLOPROVINCIALIS FOOT PROTEIN TYPE FIVE (MGFP-5)
    Lv, Ya Wei
    Zhang, Yu Jing
    Gao, Wen Ying
    Wang, Ying Juan
    PAKISTAN JOURNAL OF BOTANY, 2019, 51 (04) : 1473 - 1480
  • [2] High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Cichorium intybus L. via removing macronutrients
    Fathi, Roghayeh
    Mohebodini, Mehdi
    Chamani, Esmaeil
    INDUSTRIAL CROPS AND PRODUCTS, 2019, 128 : 572 - 580
  • [3] Optimization of Agrobacterium-mediated genetic transformation in gherkin (Cucumis anguria L.)
    Thiruvengadam, Muthu
    Jeyakumar, Jerome
    Kamaraj, Malaiyandi
    Chung, Ill-Min
    Kim, Jong-Jin
    PLANT OMICS, 2013, 6 (03) : 231 - 239
  • [4] Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes coding bacterial antigens from Mycobacterium tuberculosis
    N. A. Matvieieva
    M. Yu. Vasylenko
    A. M. Shakhovsky
    N. V. Kuchuk
    Cytology and Genetics, 2009, 43 : 94 - 98
  • [5] Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.
    Mitic, N
    Nikolic, R
    Ninkovic, S
    Miljus-Djukic, J
    Neskovic, M
    BIOLOGIA PLANTARUM, 2004, 48 (02) : 179 - 184
  • [6] Agrobacterium-mediated transformation of Phaseolus vulgaris L. using indirect organogenesis
    Collado, R.
    Bermudez-Caraballoso, I.
    Garcia, L. R.
    Veitia, N.
    Torres, D.
    Romero, C.
    Martirena-Ramirez, A.
    Angenon, G.
    SCIENTIA HORTICULTURAE, 2015, 195 : 89 - 100
  • [7] Somatic embryogenesis and Agrobacterium-mediated transformation in Bixa orellana L.
    Rangan Parimalan
    Akshatha Venugopalan
    Parvatam Giridhar
    G. A. Ravishankar
    Plant Cell, Tissue and Organ Culture (PCTOC), 2011, 105 : 317 - 328
  • [8] Somatic embryogenesis and Agrobacterium-mediated transformation in Bixa orellana L.
    Parimalan, Rangan
    Venugopalan, Akshatha
    Giridhar, Parvatam
    Ravishankar, G. A.
    PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 105 (03) : 317 - 328
  • [9] Agrobacterium-mediated transformation of millet [Setaria italica (L.)] using mature seeds
    Yuan, Jincheng
    Wei, Huiping
    Liu, Yinghui
    Dong, Zhiping
    RESEARCH JOURNAL OF BIOTECHNOLOGY, 2013, 8 (12): : 83 - 90
  • [10] Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.)
    M. Feeney
    Z. K. Punja
    In Vitro Cellular & Developmental Biology - Plant, 2003, 39 : 578 - 585
12345