Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement

被引：124

作者：

Gaurav, Kumar ^{[1
]}

Arora, Sanu ^{[1
]}

Silva, Paula ^{[2
,3
,4
]}

Sanchez-Martin, Javier ^{[5
]}

Horsnell, Richard ^{[6
]}

Gao, Liangliang ^{[2
,3
]}

Brar, Gurcharn S. ^{[7
,8
]}

Widrig, Victoria ^{[5
]}

Raupp, W. John ^{[2
,3
]}

Singh, Narinder ^{[2
,3
,37
]}

Wu, Shuangye ^{[2
,3
]}

Kale, Sandip M. ^{[9
]}

Chinoy, Catherine ^{[1
]}

Nicholson, Paul ^{[1
]}

Quiroz-Chavez, Jesus ^{[1
]}

Simmonds, James ^{[1
]}

Hayta, Sadiye ^{[1
]}

Smedley, Mark A. ^{[1
]}

Harwood, Wendy ^{[1
]}

Pearce, Suzannah ^{[1
]}

Gilbert, David ^{[1
]}

Kangara, Ngonidzashe ^{[1
]}

Gardener, Catherine ^{[1
]}

Forner-Martinez, Macarena ^{[1
]}

Liu, Jiaqian ^{[1
,10
]}

Yu, Guotai ^{[1
,38
]}

Boden, Scott A. ^{[1
,11
]}

Pascucci, Attilio ^{[1
,12
]}

Ghosh, Sreya ^{[1
]}

Hafeez, Amber N. ^{[1
]}

O'Hara, Tom ^{[1
]}

Waites, Joshua ^{[1
]}

Cheema, Jitender ^{[1
]}

Steuernagel, Burkhard ^{[1
]}

Patpour, Mehran ^{[13
]}

Justesen, Annemarie Fejer ^{[13
]}

Liu, Shuyu ^{[14
]}

Rudd, Jackie C. ^{[14
]}

Avni, Raz ^{[15
]}

Sharon, Amir ^{[15
]}

Steiner, Barbara ^{[16
]}

Kirana, Rizky Pasthika ^{[16
,17
]}

Buerstmayr, Hermann ^{[16
]}

Mehrabi, Ali A. ^{[18
]}

Nasyrova, Firuza Y. ^{[19
]}

Chayut, Noam ^{[20
]}

Matny, Oadi ^{[21
]}

Steffenson, Brian J. ^{[21
]}

Sandhu, Nitika ^{[22
]}

Chhuneja, Parveen ^{[22
]}

机构：

[1] John Innes Ctr, Norwich Res Pk, Norwich, Norfolk, England

[2] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA

[3] Kansas State Univ, Wheat Genet Resource Ctr, Manhattan, KS 66506 USA

[4] Inst Nacl Invest Agr INIA, Estn Expt Estanzuela, Programa Nacl Cult Secano, Colonia, Uruguay

[5] Univ Zurich, Dept Plant & Microbial Biol, Zurich, Switzerland

[6] NIAB, John Bingham Lab, Cambridge, England

[7] Univ Saskatchewan, Crop Dev Ctr, Dept Plant Sci, Saskatoon, SK, Canada

[8] Univ British Columbia, Fac Land & Food Syst, Vancouver, BC, Canada

[9] Leibniz Inst Plant Genet & Crop Plant Res IPK Gat, Seeland, Germany

[10] Nanjing Agr Univ, Cytogenet Inst, Natl Key Lab Crop Genet & Germplasm Enhancement, JCIC MCP, Nanjing, Peoples R China

[11] Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA, Australia

[12] Univ Bologna, Dept Agr & Food Sci, Alma Mater Studiorum, Bologna, Italy

[13] Aarhus Univ, Global Rust Reference Ctr, Dept Agroecol, Slagelse, Denmark

[14] Texas A&M AgriLife Res, Amarillo, TX USA

[15] Tel Aviv Univ, Sch Plant Sci & Food Secur, Inst Cereal Crops Improvement, Tel Aviv, Israel

[16] Univ Nat Resources & Life Sci, Inst Biotechnol Plant Prod, Dept Agrobiotechnol IFA Tulln, Vienna, Austria

[17] Univ Gadjah Mada, Fac Agr, Dept Agron, Lab Plant Breeding, Yogyakarta, Indonesia

[18] Ilam Univ, Dept Agron & Plant Breeding, Ilam, Iran

[19] Tajik Natl Acad Sci, Inst Bot Plant Physiol & Genet, Dushanbe, Tajikistan

[20] John Innes Ctr, Germplasm Resources Unit, Norwich Res Pk, Norwich, Norfolk, England

[21] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA

[22] Punjab Agr Univ, Sch Agr Biotechnol, Ludhiana, Punjab, India

[23] Commonwealth Sci & Ind Res Org CSIRO, Agr & Food, Canberra, ACT, Australia

[24] Agr Res Ctr, Field Crops Res Inst, Wheat Res Dept, Giza, Egypt

[25] Earlham Inst, Norwich Res Pk, Norwich, Norfolk, England

[26] QIAGEN Aarhus AS, Aarhus, Denmark

[27] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA

[28] Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China

[29] Sichuan Agr Univ, Triticeae Res Inst, Chengdu, Peoples R China

[30] USDA ARS Cereal Crops Res Unit, Edward T Schafer Agr Res Ctr, Fargo, ND USA

[31] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA

[32] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

[33] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[34] Helmholtz Ctr Munich, Plant Genome & Syst Biol, Neuherberg, Germany

[35] Tech Univ Munich, Fac Life Sci, Weihenstephan, Germany

[36] German Ctr Integrat Biodivers Res iDiv, Leipzig, Germany

[37] Bayer R&D Serv LLc, Kansas City, MO USA

[38] King Abdullah Univ Sci & Technol KAUST, Ctr Desert Agr, Biol & Environm Sci & Engn Div BESE, Thuwal, Saudi Arabia

[39] Int Maize & Wheat Improvement Ctr cIMMYT, Texcoco, Mexico

来源：

NATURE BIOTECHNOLOGY | 2022年 / 40卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会; 欧盟地平线“2020”; 瑞士国家科学基金会; 英国科研创新办公室; 欧洲研究理事会; 美国农业部; 美国食品与农业研究所;

关键词：

CURL-MITE; RESISTANCE GENE; HEXAPLOID WHEAT; TRITICUM-AESTIVUM; SEQUENCE; POLYMORPHISMS; PROGENITOR; DIVERSITY; EVOLUTION; TRICHOMES;

D O I：

10.1038/s41587-021-01058-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aegilops tauschii, the diploid wild progenitor of the D subgenome of bread wheat, is a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. Here we sequenced 242 Ae. tauschii accessions and compared them to the wheat D subgenome to characterize genomic diversity. We found that a rare lineage of Ae. tauschii geographically restricted to present-day Georgia contributed to the wheat D subgenome in the independent hybridizations that gave rise to modern bread wheat. Through k-mer-based association mapping, we identified discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of hexaploids incorporating diverse Ae. tauschii genomes. Exploiting the genomic diversity of the Ae. tauschii ancestral diploid genome permits rapid trait discovery and functional genetic validation in a hexaploid background amenable to breeding. Exploring the diversity of the wild progenitor of the wheat D subgenome allows for rapid trait discovery.

引用

页码：422 / +

页数：27

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