Increased concentrations of the oxidative DNA adduct 7,8-dihydro-8-oxo-2-deoxyguanosine in the germ-line of men with type 1 diabetes

被引:53
作者
Agbaje, I. M. [1 ]
McVicar, C. M. [1 ]
Schock, B. C. [2 ]
McClure, N. [2 ]
Atkinson, A. B. [3 ]
Rogers, D. [1 ]
Lewis, S. E. M. [1 ]
机构
[1] Queens Univ Belfast, Ctr Clin & Populat Sci, Inst Clin Sci, Reprod Med Res Grp, Belfast BT12 6BJ, Antrim, North Ireland
[2] Queens Univ Belfast, Inst Clin Sci, Div Med & therapeut, Belfast BT12 6BJ, Antrim, North Ireland
[3] Royal Victoria Hosp, Reg Ctr Endicrinol & Diabet, Belfast BT12 6BA, Antrim, North Ireland
关键词
diabetes mellitus; human spermatozoa; 8-hydroxydeoxyguanosine; nuclear DNA; oxidative stress;
D O I
10.1016/S1472-6483(10)60602-5
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The effects of diabetes mellitus on male reproductive health have not been clearly defined. A previous publication from this group reported significantly higher levels of nuclear DNA fragmentation and mitochondrial DNA deletions in spermatozoa from men with type 1 diabetes. This study compared semen profiles, sperm DNA fragmentation and levels of oxidative DNA modification in spermatozoa of diabetic and non-diabetic men. Semen samples from 12 non-diabetic, fertile men and 11 type 1 diabetics were obtained and subjected to conventional light microscopic semen analysis. Nuclear DNA fragmentation was assessed using an alkaline Comet assay and concentrations of 7,8-dihydro-8-oxo-2-deoxyguanosine (8-OHdG), an oxidative adduct of the purine guanosine, were assessed by high-performance liquid chromatography. Conventional semen profiles were similar in both groups, whilst spermatozoa from type I diabetics showed significantly higher levels of DNA fragmentation (44% versus 27%; P < 0.05) and concentrations of 8-OHdG (3.6 versus 2.0 molecules of 8-OHdG per 101 molecules of deoxyguanosine; P < 0.05). Furthermore, a positive correlation was observed between DNA fragmentation and concentrations of 8-OHdG per 10(5) molecules of deoxyguanosine, (r(s) = 0.7, P < 0.05). The genomic damage evident in spermatozoa of type I diabetics may have important implications for their fertility and the outcome of pregnancies fathered by these individuals.
引用
收藏
页码:401 / 409
页数:9
相关论文
共 66 条
  • [41] Paternal cigarette smoking and the risk of childhood cancer among offspring of nonsmoking mothers
    Ji, BT
    Shu, XO
    Linet, MS
    Zheng, W
    Wacholder, S
    Gao, YT
    Ying, DM
    Jin, F
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1997, 89 (03) : 238 - 244
  • [42] Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients
    Kodama, H
    Yamaguchi, R
    Fukuda, J
    Kasai, H
    Tanaka, T
    [J]. FERTILITY AND STERILITY, 1997, 68 (03) : 519 - 524
  • [43] NEW METHOD OF EVALUATING SPERM MORPHOLOGY WITH PREDICTIVE VALUE FOR HUMAN INVITRO FERTILIZATION
    KRUGER, TF
    ACOSTA, AA
    SIMMONS, KF
    SWANSON, RJ
    MATTA, JF
    VEECK, LL
    MORSHEDI, M
    BRUGO, S
    [J]. UROLOGY, 1987, 30 (03) : 248 - 251
  • [44] Oxidative DNA damage in human sperm influences time to pregnancy
    Loft, S
    Kold-Jensen, T
    Hjollun, NH
    Giwercman, A
    Gyllemborg, J
    Ernst, E
    Olsen, J
    Scheike, T
    Poulsen, HE
    Bonde, JP
    [J]. HUMAN REPRODUCTION, 2003, 18 (06) : 1265 - 1272
  • [45] PRESENCE OF ENDOGENOUS NICKS IN DNA OF EJACULATED HUMAN SPERMATOZOA AND ITS RELATIONSHIP TO CHROMOMYCIN A(3) ACCESSIBILITY
    MANICARDI, GC
    BIANCHI, PG
    PANTANO, S
    AZZONI, P
    BIZZARO, D
    BIANCHI, U
    SAKKAS, D
    [J]. BIOLOGY OF REPRODUCTION, 1995, 52 (04) : 864 - 867
  • [46] X-RAY-INDUCED AND MITOMYCIN-C (MMC)-INDUCED CHROMOSOME-ABERRATIONS IN SPERMIOGENIC GERM-CELLS AND THE REPAIR CAPACITY OF MOUSE EGGS FOR THE X-RAY AND MMC DAMAGE
    MATSUDA, Y
    SEKI, N
    UTSUGITAKEUCHI, T
    TOBARI, I
    [J]. MUTATION RESEARCH, 1989, 211 (01): : 65 - 75
  • [47] REPAIR CAPACITY OF FERTILIZED MOUSE EGGS FOR X-RAY-DAMAGE INDUCED IN SPERM AND MATURE OOCYTES
    MATSUDA, Y
    TOBARI, I
    [J]. MUTATION RESEARCH, 1989, 210 (01): : 35 - 47
  • [48] Does the increase of 8-hydroxydeoxyguanosine lead to poor sperm quality?
    Ni, ZY
    Liu, YQ
    Shen, HM
    Chia, SE
    Ong, CN
    [J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1997, 381 (01) : 77 - 82
  • [49] Sperm DNA integrity and male infertility
    O'Brien, J
    Zini, A
    [J]. UROLOGY, 2005, 65 (01) : 16 - 22
  • [50] Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay
    Oger, I
    Da Cruz, C
    Panteix, G
    Menezo, Y
    [J]. ZYGOTE, 2003, 11 (04) : 367 - 371