Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo

被引:101
作者
Butko, Emerald [1 ]
Distel, Martin [1 ]
Pouget, Claire [1 ]
Weijts, Bart [1 ]
Kobayashi, Isao [1 ]
Ng, Kevin [1 ]
Mosimann, Christian [2 ]
Poulain, Fabienne E. [3 ]
McPherson, Adam [3 ]
Ni, Chih-Wen [4 ]
Stachura, David L. [1 ]
Del Cid, Natasha [1 ]
Espin-Palazon, Raquel [1 ]
Lawson, Nathan D.
Dorsky, Richard [3 ]
Clements, Wilson K. [1 ,5 ]
Traver, David [1 ,6 ]
机构
[1] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
[2] Univ Zurich, Inst Mol Life Sci, Zurich, Switzerland
[3] Univ Utah, Dept Neurobiol & Anat, Salt Lake City, UT 84132 USA
[4] Univ Massachusetts, Worcester, MA 01605 USA
[5] St Jude Childrens Res Hosp, Dept Hematol, Memphis, TN 38105 USA
[6] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA
来源
DEVELOPMENT | 2015年 / 142卷 / 06期
基金
美国国家卫生研究院;
关键词
Hematopoietic stem cell; Hemogenic endothelium; Subfunctionalization; Gata2; Notch; HEMATOPOIETIC STEM-CELLS; TRANSCRIPTION FACTOR; DEFINITIVE HEMATOPOIESIS; DISTINCT POPULATIONS; VASCULAR DEVELOPMENT; AORTIC ENDOTHELIUM; RUNX1; EXPRESSION; CIS-ELEMENT; WILD-TYPE; PROGENITORS;
D O I
10.1242/dev.119180
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The adult blood system is established by hematopoietic stem cells (HSCs), which arise during development from an endothelial-to-hematopoietic transition of cells comprising the floor of the dorsal aorta. Expression of aortic runx1 has served as an early marker of HSC commitment in the zebrafish embryo, but recent studies have suggested that HSC specification begins during the convergence of posterior lateral plate mesoderm (PLM), well before aorta formation and runx1 transcription. Further understanding of the earliest stages of HSC specification necessitates an earlier marker of hemogenic endothelium. Studies in mice have suggested that GATA2 might function at early stages within hemogenic endothelium. Two orthologs of Gata2 exist in zebrafish: gata2a and gata2b. Here, we report that gata2b expression initiates during the convergence of PLM, becoming restricted to emerging HSCs. We observe Notch-dependent gata2b expression within the hemogenic subcompartment of the dorsal aorta that is in turn required to initiate runx1 expression. Our results indicate that Gata2b functions within hemogenic endothelium from an early stage, whereas Gata2a functions more broadly throughout the vascular system.
引用
收藏
页码:1050 / 1061
页数:12
相关论文
共 80 条
[1]   Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair [J].
Auer, Thomas O. ;
Duroure, Karine ;
De Cian, Anne ;
Concordet, Jean-Paul ;
Del Bene, Filippo .
GENOME RESEARCH, 2014, 24 (01) :142-153
[2]   Eosinophils in the zebrafish: prospective isolation, characterization, and eosinophilia induction by helminth determinants [J].
Balla, Keir M. ;
Lugo-Villarino, Geanncarlo ;
Spitsbergen, Jan M. ;
Stachura, David L. ;
Hu, Yan ;
Banuelos, Karina ;
Romo-Fewell, Octavio ;
Aroian, Raffi V. ;
Traver, David .
BLOOD, 2010, 116 (19) :3944-3954
[3]   The mouse Runx1+23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters [J].
Bee, Thomas ;
Ashley, Emma L. K. ;
Bickley, Sorrel R. B. ;
Jarratt, Andrew ;
Li, Pik-Shan ;
Sloane-Stanley, Jackie ;
Goettgens, Berthold ;
de Bruijn, Marella F. T. R. .
BLOOD, 2009, 113 (21) :5121-5124
[4]   CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis [J].
Bertrand, Julien Y. ;
Kim, Albert D. ;
Teng, Shutian ;
Traver, David .
DEVELOPMENT, 2008, 135 (10) :1853-1862
[5]   Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo [J].
Bertrand, Julien Y. ;
Kim, Albert D. ;
Violette, Emily P. ;
Stachura, David L. ;
Cisson, Jennifer L. ;
Traver, David .
DEVELOPMENT, 2007, 134 (23) :4147-4156
[6]   Haematopoietic stem cells derive directly from aortic endothelium during development [J].
Bertrand, Julien Y. ;
Chi, Neil C. ;
Santoso, Buyung ;
Teng, Shutian ;
Stainier, Didier Y. R. ;
Traver, David .
NATURE, 2010, 464 (7285) :108-U120
[7]   Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta [J].
Boisset, Jean-Charles ;
Clapes, Thomas ;
Klaus, Anna ;
Papazian, Natalie ;
Onderwater, Jos ;
Mommaas-Kienhuis, Mieke ;
Cupedo, Tom ;
Robin, Catherine .
BLOOD, 2015, 125 (03) :465-469
[8]   In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium [J].
Boisset, Jean-Charles ;
van Cappellen, Wiggert ;
Andrieu-Soler, Charlotte ;
Galjart, Niels ;
Dzierzak, Elaine ;
Robin, Catherine .
NATURE, 2010, 464 (7285) :116-U131
[9]   Insights into early vasculogenesis revealed by expression of the ETS-domain transcription factor Fli-1 in wild-type and mutant zebrafish embryos [J].
Brown, LA ;
Rodaway, ARF ;
Schilling, TF ;
Jowett, T ;
Ingham, PW ;
Patient, RK ;
Sharrocks, AD .
MECHANISMS OF DEVELOPMENT, 2000, 90 (02) :237-252
[10]   Hematopoietic stem cell fate is established by the Notch-Runx pathway [J].
Burns, CE ;
Traver, D ;
Mayhall, E ;
Shepard, JL ;
Zon, LI .
GENES & DEVELOPMENT, 2005, 19 (19) :2331-2342