Monomolecular films of cholesterol oxidase and S-Layer proteins

被引：12

作者：

Ferraz, Helen Conceicao ^{[1
]}

Guimaraes, Juliana Aguilar ^{[1
]}

Moitinho Alves, Tito Livio ^{[1
]}

Leopoldo Constantino, Carlos Jose ^{[2
]}

机构：

[1] Univ Fed Rio de Janeiro, Chem Engn Program, BR-21941972 Rio De Janeiro, Brazil

[2] Univ Estadual Paulista, Dept Phys Chem & Biol, BR-19060900 Presidente Prudente, SP, Brazil

来源：

APPLIED SURFACE SCIENCE | 2011年 / 257卷 / 15期

关键词：

Cholesterol; Cholesterol oxidase; S-Layer proteins; Monolayer; Biosensor; LANGMUIR-BLODGETT; BIOSENSOR;

D O I：

10.1016/j.apsusc.2011.01.143

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Cholesterol oxidase (ChOx) is a flavoenzyme that catalyzes the oxidation of cholesterol to cholest-5-en-3-one and subsequently the isomerization to cholest-4-en-3-one. ChOx has been very commonly studied as the detection element in cholesterol biosensors. In the biosensor development field, a relatively new approach is the use of crystalline bacterial cell surface layers, known as S-Layer proteins. These proteins exhibit the ability of self-assembling at surfaces, opening a vast spectrum of applications, both in basic and applied researches. In our study, monomolecular films of ChOx and mixed films of ChOx/S-Layer proteins and DPPC/S-Layer proteins were produced using the Langmuir technique. Characterization of the films was performed by means of surface pressure-molecular area (pi-A) isotherms. Stable monolayers were obtained, which means that they can be transferred to solid substrates by Langmuir-Blodgett technique. Mixed monolayers showed an ideal like behavior. (C) 2011 Elsevier B. V. All rights reserved.

引用

页码：6535 / 6539

页数：5

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